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Rabbit Recombinant Monoclonal MIF antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

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Images

Western blot - Anti-MIF antibody [EPR12463] (AB175189), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (AB175189), expandable thumbnail
  • Western blot - Anti-MIF antibody [EPR12463] (AB175189), expandable thumbnail
  • Western blot - Anti-MIF antibody [EPR12463] (AB175189), expandable thumbnail
  • Immunoprecipitation - Anti-MIF antibody [EPR12463] (AB175189), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Mouse
Expected
Tested
Not recommended
Expected
Rat
Expected
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
1/50
Notes

For unpurified use at 1/30.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/500 - 1/1000.

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/500 - 1/1000.

Species
Human
Dilution info
1/1000 - 1/10000
Notes

For unpurified use at 1/500 - 1/1000.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/140
Notes

For unpurified use at 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494).

Alternative names

GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF

Recommended products

Rabbit Recombinant Monoclonal MIF antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12463
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.

Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

Associated diseases and disorders

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Western blot - Anti-MIF antibody [EPR12463] (ab175189)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: MIF knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: HepG2 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab175189 observed at 12 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab175189 was shown to specifically react with MIF in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when knockout cells were examined. Wild-type and MIF knockout samples were subjected to SDS-PAGE. ab175189 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/2,0000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MIF antibody [EPR12463] (ab175189)

    Predicted band size: 12 kDa

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)

    Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with unpurified ab175189 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Western blot - Anti-MIF antibody [EPR12463] (ab175189)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution

    Lane 1: Y79 cell lysate at 20 µg/mL

    Lane 2: Mouse brain tissue lysate at 20 µg/mL

    Lane 3: Rat brain tissue lysate at 20 µg/mL

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Western blot - Anti-MIF antibody [EPR12463] (ab175189)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-MIF antibody [EPR12463] (ab175189) at 1/2000 dilution

    Lane 1: Y79 cell lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

    ab175189 (unpurified) at 1/30 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Western blot - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Western blot - Anti-MIF antibody [EPR12463] (ab175189)

    All lanes: Western blot - Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution

    Lane 1: Y79 lysates at 10 µg

    Lane 2: THP-1 lysates at 10 µg

    Lane 3: Molt-4 lysates at 10 µg

    Lane 4: Human fetal brain lysates at 10 µg

    Predicted band size: 12 kDa

  • Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

    ab175189 (purified) at 1/50 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)

    Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with purified ab175189 at 1/140 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (ab175189)

    Intracellular flow cytometric analysis of permeabilized Molt-4 cells using unpurified ab175189 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

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