JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB175189

Anti-MIF antibody [EPR12463]

Be the first to review this product! Submit a review

|

(15 Publications)

Rabbit Recombinant Monoclonal MIF antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

View Alternative Names

GLIF, MMIF, MIF, Macrophage migration inhibitory factor, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, GIF

9 Images
Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (AB175189)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (AB175189)

Intracellular flow cytometric analysis of permeabilized Molt-4 cells using unpurified ab175189 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (AB175189)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (AB175189)

Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with purified ab175189 at 1/140 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (AB175189)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] (AB175189)

Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with unpurified ab175189 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

Immunoprecipitation - Anti-MIF antibody [EPR12463] (AB175189)
  • IP

Lab

Immunoprecipitation - Anti-MIF antibody [EPR12463] (AB175189)

ab175189 (unpurified) at 1/30 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Immunoprecipitation - Anti-MIF antibody [EPR12463] (AB175189)
  • IP

Lab

Immunoprecipitation - Anti-MIF antibody [EPR12463] (AB175189)

ab175189 (purified) at 1/50 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-MIF antibody [EPR12463] (ab175189)

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Western blot - Anti-MIF antibody [EPR12463] (AB175189)
  • WB

Supplier Data

Western blot - Anti-MIF antibody [EPR12463] (AB175189)

All lanes:

Western blot - Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution

Lane 1:

Y79 lysates at 10 µg

Lane 2:

THP-1 lysates at 10 µg

Lane 3:

Molt-4 lysates at 10 µg

Lane 4:

Human fetal brain lysates at 10 µg

Predicted band size: 12 kDa

false

Western blot - Anti-MIF antibody [EPR12463] (AB175189)
  • WB

Unknown

Western blot - Anti-MIF antibody [EPR12463] (AB175189)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-MIF antibody [EPR12463] (ab175189) at 1/1000 dilution

Lane 1:

Y79 cell lysate at 20 µg/mL

Lane 2:

Mouse brain tissue lysate at 20 µg/mL

Lane 3:

Rat brain tissue lysate at 20 µg/mL

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Western blot - Anti-MIF antibody [EPR12463] (AB175189)
  • WB

Lab

Western blot - Anti-MIF antibody [EPR12463] (AB175189)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : MIF knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HepG2 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab175189 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab175189 was shown to specifically react with MIF in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when knockout cells were examined. Wild-type and MIF knockout samples were subjected to SDS-PAGE. ab175189 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/2,0000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MIF antibody [EPR12463] (ab175189)

Predicted band size: 12 kDa

false

Western blot - Anti-MIF antibody [EPR12463] (AB175189)
  • WB

Lab

Western blot - Anti-MIF antibody [EPR12463] (AB175189)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-MIF antibody [EPR12463] (ab175189) at 1/2000 dilution

Lane 1:

Y79 cell lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

  • HRP

    HRP Anti-MIF antibody [EPR12463]

  • Carrier free

    Anti-MIF antibody [EPR12463] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12463

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB227073

Anti-MIF antibody

Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody (AB227073)

  • 0
  • 0
Primary Antibodies

AB36146

Anti-MIF antibody

Western blot - Anti-MIF antibody (AB36146)

  • 4
  • 4
Primary Antibodies

AB65869

Anti-MIF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB65869)

  • 5
  • 4
Primary Antibodies

AB34644

Anti-MIF antibody

Western blot - Anti-MIF antibody (AB34644)

  • 0
  • 0
Primary Antibodies

AB7207

Anti-MIF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody (AB7207)

  • 5
  • 11
Primary Antibodies

AB187064

Anti-MIF antibody [EPR18149-128]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] (AB187064)

  • 0
  • 0
Primary Antibodies

AB226166

Anti-MIF antibody [EPR18149-128] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-128] - BSA and Azide free (AB226166)

  • 4
  • 1
Primary Antibodies

AB189290

Anti-MIF antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody - C-terminal (AB189290)

  • 0
  • 0
Primary Antibodies

AB55445

Anti-MIF antibody [2A10-4D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIF antibody [2A10-4D3] (AB55445)

  • 5
  • 2
Primary Antibodies

AB176565

Anti-MIF antibody [EPR12462]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR12462] (AB176565)

  • 5
  • 2

Complementary Products

Assay Kits

AB209885

Mouse MIF ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MIF ELISA Kit (AB209885)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 49
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p><strong>For unpurified use at 1/30.</strong></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500 - 1/1000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/140", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/50. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500 - 1/1000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p><strong>For unpurified use at 1/500 - 1/1000.</strong></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.
Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed : 15908412, PubMed : 17443469, PubMed : 23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed : 11439086, PubMed : 17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed : 11439086, PubMed : 17526494).
See full target information MIF
style
left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40846326

2025

Multiomics integration analysis identifies tumor cell-derived MIF as a therapeutic target and potentiates anti-PD-1 therapy in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weidong Chen,Yan Liao,Hao Yao,Yutong Zou,Ji Fang,Jiongfeng Zhang,Zehao Guo,Jian Tu,Junkai Chen,Zijun Huo,Lili Wen,Xianbiao Xie

Molecular neurobiology 61:10481-10499 PubMed38743209

2024

MicroRNA-451 Regulates Angiogenesis in Intracerebral Hemorrhage by Targeting Macrophage Migration Inhibitory Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Bai,Ge Zhang,Shiling Chen,Xuan Wu,Jiarui Li,Jingxuan Wang,Danyang Chen,Xia Liu,Jiahui Wang,Yuanwei Li,Yingxin Tang,Zhouping Tang

Molecular medicine reports 29: PubMed38639187

2024

Network pharmacology combined with experimental validation to investigate the effect of Rongjin Niantong Fang on chondrocyte apoptosis in knee osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Chen,Ting Zhang,Qingqing Luo,Ruyi Wang,Yuting Dai,Zhenyuan Chen,Chutian Zhang,Xuzheng Chen,Guangwen Wu

PLoS biology 19:e3001121 PubMed33661886

2021

CD74 is a regulator of hematopoietic stem cell maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Shirly Becker-Herman,Milena Rozenberg,Carmit Hillel-Karniel,Naama Gil-Yarom,Mattias P Kramer,Avital Barak,Lital Sever,Keren David,Lihi Radomir,Hadas Lewinsky,Michal Levi,Gilgi Friedlander,Richard Bucala,Amnon Peled,Idit Shachar

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:2002874 PubMed33898171

2021

OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyu Luo,Xiaowei Wu,Pengfei Zhao,Yabing Nan,Wan Chang,Xiaolin Zhu,Dan Su,Zhihua Liu

Nature communications 10:903 PubMed30796225

2019

Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Kurelac,Luisa Iommarini,Renaud Vatrinet,Laura Benedetta Amato,Monica De Luise,Giulia Leone,Giulia Girolimetti,Nikkitha Umesh Ganesh,Victoria Louise Bridgeman,Luigi Ombrato,Marta Columbaro,Moira Ragazzi,Lara Gibellini,Manuela Sollazzo,Rene Gunther Feichtinger,Silvia Vidali,Maurizio Baldassarre,Sarah Foriel,Michele Vidone,Andrea Cossarizza,Daniela Grifoni,Barbara Kofler,Ilaria Malanchi,Anna Maria Porcelli,Giuseppe Gasparre

The Journal of biological chemistry 293:19740-19760 PubMed30366984

2018

Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rudranil De,Souvik Sarkar,Somnath Mazumder,Subhashis Debsharma,Asim Azhar Siddiqui,Shubhra Jyoti Saha,Chinmoy Banerjee,Shiladitya Nag,Debanjan Saha,Saikat Pramanik,Uday Bandyopadhyay

European review for medical and pharmacological sciences 22:4908-4916 PubMed30070326

2018

MiR-608 exerts tumor suppressive function in lung adenocarcinoma by directly targeting MIF.

Applications

WB

Species

Unspecified reactive species

H-X Yu,X-M Wang,X-D Han,B-F Cao

International journal of molecular medicine 41:1062-1068 PubMed29207023

2017

Macrophage migration inhibitory factor promotes Warburg effect via activation of the NF‑κB/HIF‑1α pathway in lung cancer.

Applications

WB

Species

Unspecified reactive species

Jun Li,Junhua Zhang,Fengjiao Xie,Jiangzhou Peng,Xu Wu

Scientific reports 7:6361 PubMed28743960

2017

NADPH oxidase 4 is required for the generation of macrophage migration inhibitory factor and host defense against Toxoplasma gondii infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hye Kim,Jina Lee,Su-Jin Bae,Yeeun Kim,Byung-Joon Park,Jae-Won Choi,Jaeyul Kwon,Guang-Ho Cha,Heon Jong Yoo,Eun-Kyeong Jo,Yun Soo Bae,Young-Ha Lee,Jae-Min Yuk
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com