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Rabbit Recombinant Monoclonal MIF antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-MIF antibody [EPR12463] - BSA and Azide free (AB183302), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (AB183302), expandable thumbnail
  • Immunoprecipitation - Anti-MIF antibody [EPR12463] - BSA and Azide free (AB183302), expandable thumbnail
  • Immunoprecipitation - Anti-MIF antibody [EPR12463] - BSA and Azide free (AB183302), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (AB183302), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Rat
Expected
Expected
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (PubMed:15908412, PubMed:17443469, PubMed:23776208). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (PubMed:15908412, PubMed:17443469, PubMed:23776208). Counteracts the anti-inflammatory activity of glucocorticoids (PubMed:15908412, PubMed:17443469, PubMed:23776208). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:11439086, PubMed:17526494). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:11439086, PubMed:17526494).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MIF antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR12463

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab183302 is the carrier-free version of Anti-MIF antibody [EPR12463] ab175189.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.

Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

Associated diseases and disorders

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302), expandable thumbnail

    Western blot - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: MIF knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: HepG2 whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-MIF antibody [EPR12463] ab175189 observed at 12 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    Anti-MIF antibody [EPR12463] ab175189 was shown to specifically react with MIF in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when knockout cells were examined. Wild-type and MIF knockout samples were subjected to SDS-PAGE. Anti-MIF antibody [EPR12463] ab175189 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/2,0000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MIF antibody [EPR12463] ab175189).

    All lanes: Western blot - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302)

    Predicted band size: 12 kDa

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302)

    Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with unpurified Anti-MIF antibody [EPR12463] ab175189 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MIF antibody [EPR12463] ab175189).

  • Immunoprecipitation - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302), expandable thumbnail

    Immunoprecipitation - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302)

    Anti-MIF antibody [EPR12463] ab175189 (unpurified) at 1/30 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MIF antibody [EPR12463] ab175189).

    All lanes: Immunoprecipitation - Anti-MIF antibody [EPR12463] (Anti-MIF antibody [EPR12463] ab175189)

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Immunoprecipitation - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302), expandable thumbnail

    Immunoprecipitation - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302)

    Anti-MIF antibody [EPR12463] ab175189 (purified) at 1/50 immunoprecipitating MIF in human fetal brain. For western blotting, a Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MIF antibody [EPR12463] ab175189).

    All lanes: Immunoprecipitation - Anti-MIF antibody [EPR12463] (Anti-MIF antibody [EPR12463] ab175189)

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302)

    Intracellular Flow Cytometry analysis of THP-1 cells labelling MIF with purified Anti-MIF antibody [EPR12463] ab175189 at 1/140 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary anitbody. A rabbit monoclonal IgG was used as the isotype control (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MIF antibody [EPR12463] ab175189).

  • Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MIF antibody [EPR12463] - BSA and Azide free (ab183302)

    Intracellular flow cytometric analysis of permeabilized Molt-4 cells using unpurified Anti-MIF antibody [EPR12463] ab175189 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MIF antibody [EPR12463] ab175189).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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