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Rabbit Recombinant Monoclonal MIF antibody. Carrier free. Suitable for sELISA, WB, ICC/IF and reacts with Mouse, Human, Rat samples.

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Images

Sandwich ELISA - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (AB242509), expandable thumbnail
  • Western blot - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (AB242509), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (AB242509), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (AB242509), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISAWBICC/IF
Human
Predicted
Tested
Not recommended
Mouse
Expected
Tested
Tested
Rat
Predicted
Tested
Tested

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens (By similarity). The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense (By similarity). Counteracts the anti-inflammatory activity of glucocorticoids (By similarity). Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known (PubMed:10933783, PubMed:16780921, PubMed:19188446). It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity (PubMed:10933783, PubMed:16780921, PubMed:19188446).

Alternative names

Macrophage migration inhibitory factor, MIF, Delayed early response protein 6, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase, DER6, GIF, Mif

Recommended products

Rabbit Recombinant Monoclonal MIF antibody. Carrier free. Suitable for sELISA, WB, ICC/IF and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR18149-132
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab242509 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MIF. The recommended pair for sandwich ELISA is:
Capture: ab242509, Mouse MIF Capture Antibody (unconjugated)
Detector: Anti-MIF antibody [EPR18149-202] - BSA and Azide free (Detector) ab242758, Mouse MIF Detector Antibody (unconjugated)
The reference range value is 125 - 8000 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Macrophage migration inhibitory factor (MIF) also known as glycosylation-inhibiting factor is a protein with a molecular mass of approximately 12.5 kDa. It plays a mechanistic role as a cytokine that regulates immune responses specifically influencing macrophage behavior. MIF expresses in various cell types including immune and epithelial cells. It gets secreted by activated lymphocytes and macrophages pointing to its involvement in the inflammatory process.

Biological function summary

MIF acts as an upstream regulator of innate immunity and exerts broad influence on cytokine release. MIF elevates pro-inflammatory cytokines like TNF-alpha and IL-1beta promoting an immune response. It does not typically form part of a complex but interacts with cell surface receptors like CD74 triggering intracellular signaling pathways. These interactions illustrate MIF as a regulator of immune cell recruitment and activation.

Pathways

MIF participates in the glucocorticoid receptor regulatory pathway and MAP kinase pathway. It shows interaction with JNK and ERK1/2 proteins which further map into important cellular reactions like stress response and cell proliferation. By integrating into these pathways MIF influences cell survival proliferation and inflammatory cascades emphasizing its role in immune regulation.

Associated diseases and disorders

MIF links to inflammatory diseases such as rheumatoid arthritis and sepsis. It influences disease progression by interacting with proteins like TNF-alpha and IL-6 contributing to inflammatory damage. Elevated MIF levels often correlate with disease severity in rheumatoid arthritis acting as a potential target for therapy. Furthermore sepsis demonstrates a similar pattern where MIF modulation can impact patient outcomes underlining its relevance in clinical investigations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509), expandable thumbnail

    Sandwich ELISA - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509)

    Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse MIF ELISA Kit ab209885).

  • Western blot - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509), expandable thumbnail

    Western blot - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at a 1/200000 dilution.

    In Western blot, ab242509 was shown to bind specifically to MIF. Target of interest was observed at 13 kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in MIF knockout cell line (lane 2).

    Exposure time: Lane 1-4: 180 seconds
    Lane 5-8: 15 seconds

    All lanes: Western blot - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

    Lane 2: MIF knockout HAP1 whole cell lysate at 20 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 5: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 7: J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate at 20 µg

    Lane 8: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 13 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling MIF with ab242509 at 1/100 (20.9 µg/ml) dilution followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MIF antibody [EPR18149-132] - BSA and Azide free (Capture) (ab242509)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 ( rat glial tumor glial cell) cells labelling MIF with ab242509 at 1/100 (10.45 µg/ml) dilution followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in C6 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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