JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB198834

Anti-MIG-6 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal MIG-6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MIG6, ERRFI1, ERBB receptor feedback inhibitor 1, Mitogen-inducible gene 6 protein, MIG-6

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIG-6 antibody (AB198834)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIG-6 antibody (AB198834)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling MIG-6 with ab198834 at 1/35 dilution.

Western blot - Anti-MIG-6 antibody (AB198834)
  • WB

Supplier Data

Western blot - Anti-MIG-6 antibody (AB198834)

8% SDS-PAGE

All lanes:

Western blot - Anti-MIG-6 antibody (ab198834) at 1/400 dilution

Lane 1:

Human brain malignant glioma tissue lysate at 40 µg

Lane 2:

HepG2 cell lysate at 40 µg

Predicted band size: 51 kDa

false

Exposure time: 5min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB227944

Anti-MIG-6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIG-6 antibody (AB227944)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB215036

Anti-KLF4 antibody [EPR19590]

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] (AB215036)

  • 5
  • 5
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB40815

Anti-EGFR (phospho Y1068) antibody [EP774Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)

  • 4
  • 14
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIG-6 also known as Gene 33 or ERRFI1 is a protein that functions as an adaptor/scaffold. It has an approximate mass of 70 kilodaltons and is found predominantly in epithelial tissues. MIG-6 acts as a negative regulator of the epidermal growth factor receptor (EGFR) signaling pathway. This regulation role involves binding to the tyrosine kinase domain of the EGFR which leads to a decrease in its catalytic activity. Besides its presence in epithelial tissues MIG-6 is also expressed in a variety of other tissue types including the liver and pancreas.
Biological function summary

MIG-6 plays a significant role in the regulation of cell proliferation differentiation and apoptosis. It is a part of a larger signal transduction network which is essential for controlling cellular responses to external stimuli. By acting as a brake on EGFR signaling MIG-6 helps to balance cell activities to prevent excessive growth and proliferation. The protein's impact extends to influencing the behavior of other signaling molecules within the cell contributing to meditative roles in maintaining cellular harmony.

Pathways

MIG-6 is integral to the EGFR signaling pathway and is closely associated with the MAPK (mitogen-activated protein kinase) pathway. Its regulatory function affects downstream signaling events that involve proteins like RAF MEK and ERK which are all critical in cell growth and survival. By interacting with these pathways MIG-6 modulates signal transduction cascades which allows for controlled cellular functions and responses. All these connections make it an important node for intracellular communication and pathway intersections.

Deficiencies or dysregulation of MIG-6 have been linked to cancer particularly in tumors that have heightened EGFR activity. The protein's ability to suppress EGFR signaling is essential in preventing unchecked cell proliferation that can lead to tumor growth. Additionally research has connected altered levels of MIG-6 with inflammatory disorders. Abnormal interactions between MIG-6 and EGFR can exacerbate inflammatory responses contributing to various chronic inflammatory conditions. Understanding these associations helps in the development of targeted therapies for conditions with altered EGFR signaling.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development (By similarity).
See full target information ERRFI1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 20:571 PubMed32552717

2020

Suppression of Mig-6 overcomes the acquired EGFR-TKI resistance of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Da Hyun Kang,Sung Soo Jung,Min-Kyung Yeo,Da Hye Lee,Geon Yoo,Sang Yeon Cho,In-Jae Oh,Ju-Ock Kim,Hee Sun Park,Chaeuk Chung,Jeong Eun Lee

EMBO reports 19: PubMed29335246

2018

Differential roles of ERRFI1 in EGFR and AKT pathway regulation affect cancer proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Junmei Cairns,Brooke L Fridley,Gregory D Jenkins,Yongxian Zhuang,Jia Yu,Liewei Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com