Anti-MIG-6 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIG-6 antibody (AB227944)

Paraffin-embedded mouse placenta tissue stained for MIG-6 using ab227944 at 1/500 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-MIG-6 antibody (AB227944)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-MIG-6 antibody (ab227944) at 1/1000 dilution

All lanes:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 51 kDa

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• WB

Lab

Western blot - Anti-MIG-6 antibody (AB227944)

Western blot : Anti-ERRFI1 antibody (ab227944) staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab227944 was shown to bind specifically to ERRFI1. A band was observed at 51 kDa in wild-type A549 cell lysates with no signal observed at this size in ERRFI1 knockout cell line. To generate this image, wild-type and ERRFI1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MIG-6 antibody (ab227944) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ERRFI1 knockout A549 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 51 kDa

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