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AB17787

Anti-Milk Fat Globule 1 antibody [SPM291]

3

(1 Review)

|

(6 Publications)

Mouse Monoclonal Milk Fat Globule 1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Lactadherin, Breast epithelial antigen BA46, HMFG, MFGM, Milk fat globule-EGF factor 8, SED1, MFG-E8, MFGE8

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

IHC image of ab17787 staining in human carinoma Renal Cell formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

IHC image of ab17787 staining in human carinoma breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Milk Fat Globule 1 antibody [SPM291] (AB17787)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Milk Fat Globule 1 with ab18878 at 1 ug/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM291

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1.00000-2.00000 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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AB235638

Human MFGE8 ELISA Kit

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0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.09% Sodium azide Constituents: BSA, 0.0268% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.. Medin. Main constituent of aortic medial amyloid.
See full target information MFGE8
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Development (Cambridge, England) 145: PubMed30093551

2018

Minor spliceosome inactivation causes microcephaly, owing to cell cycle defects and death of self-amplifying radial glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marybeth Baumgartner,Anouk M Olthof,Gabriela S Aquino,Katery C Hyatt,Christopher Lemoine,Kyle Drake,Nikita Sturrock,Nhut Nguyen,Sahar Al Seesi,Rahul N Kanadia

Nature communications 9:3133 PubMed30087345

2018

Regulation of striatal cells and goal-directed behavior by cerebellar outputs.

Applications

Unspecified application

Species

Unspecified reactive species

Le Xiao,Caroline Bornmann,Laetitia Hatstatt-Burklé,Peter Scheiffele

Reproduction (Cambridge, England) 152:225-33 PubMed27340235

2016

MFGE8 regulates TGF-β-induced epithelial mesenchymal transition in endometrial epithelial cells in vitro.

Applications

ICC/IF, WB

Species

Human, Human

Liang Yu,Rong Hu,Claretta Sullivan,R James Swanson,Sergio Oehninger,Ying-Pu Sun,Silvina Bocca

The Journal of biological chemistry 289:24560-72 PubMed25006249

2014

Polarization of prostate cancer-associated macrophages is induced by milk fat globule-EGF factor 8 (MFG-E8)-mediated efferocytosis.

Applications

IHC-P

Species

Human

Fabiana N Soki,Amy J Koh,Jacqueline D Jones,Yeo Won Kim,Jinlu Dai,Evan T Keller,Kenneth J Pienta,Kamran Atabai,Hernan Roca,Laurie K McCauley

Fertility and sterility 101:552-9 PubMed24262600

2013

Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells.

Applications

Unspecified application

Species

Human

Liang Yu,Sandra Anderson,Sergio Oehninger,Silvina Bocca

Molecular human reproduction 17:360-71 PubMed21177637

2010

Expression of milk fat globule EGF-factor 8 (MFG-E8) mRNA and protein in the human endometrium and its regulation by prolactin.

Applications

WB, IHC-Fr, IHC-P, ICC/IF

Species

Human, Human, Human, Human

Anahi Franchi,Silvina Bocca,Sandra Anderson,Ryan Riggs,Sergio Oehninger
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
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