Anti-Mimitin antibody [EP15279] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] - BSA and Azide free (AB251134)

This data was developed using ab192267, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Mimitin with ab192267 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
Negative controls, ab192267 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - BSA and Azide free (AB251134)

This data was developed using ab192267, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Mimitin antibody [EP15279] - BSA and Azide free (AB251134)

This data was developed using ab192267, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (<a href='/en-us/products/primary-antibodies/mimitin-antibody-ep15279-c-terminal-ab192267'>ab192267</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

HEL cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] - BSA and Azide free (AB251134)

This data was developed using ab192267, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Mimitin antibody [EP15279] - BSA and Azide free (AB251134)

This data was developed using ab192267, the same antibody clone in a different buffer formulation.

Western blot analysis of Mimitin in immunoprecipitation pellets from MDA-MB-435 lysate. ab192267 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Mimitin antibody [EP15279] - C-terminal (<a href='/en-us/products/primary-antibodies/mimitin-antibody-ep15279-c-terminal-ab192267'>ab192267</a>)

Predicted band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-Mimitin antibody [EP15279] - BSA and Azide free (AB251134)

This data was developed using ab192267, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (<a href='/en-us/products/primary-antibodies/mimitin-antibody-ep15279-c-terminal-ab192267'>ab192267</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Mouse kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 20 kDa

false

• WB

Supplier Data

Western blot - Anti-Mimitin antibody [EP15279] - BSA and Azide free (AB251134)

This data was developed using ab192267, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Mimitin antibody [EP15279] - C-terminal (<a href='/en-us/products/primary-antibodies/mimitin-antibody-ep15279-c-terminal-ab192267'>ab192267</a>) at 1/10000 dilution

All lanes:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 20 kDa,82 kDa

Observed band size: 20 kDa

false