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Rabbit Recombinant Monoclonal Mint-1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (AB251021), expandable thumbnail
  • Western blot - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (AB251021), expandable thumbnail
  • Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (AB251021), expandable thumbnail
  • Western blot - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (AB251021), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor protein (APP) and hence formation of APP-beta. Component of the LIN-10-LIN-2-LIN-7 complex, which associates with the motor protein KIF17 to transport vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules (By similarity).

Alternative names

MINT1, X11, APBA1, Amyloid-beta A4 precursor protein-binding family A member 1, Adapter protein X11alpha, Neuron-specific X11 protein, Neuronal Munc18-1-interacting protein 1, Mint-1

Recommended products

Rabbit Recombinant Monoclonal Mint-1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15941
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251021 is the carrier-free version of Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021)

    This data was developed using Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded human transitional cell carcinoma of the bladder labeling Mint-1 with Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359 at 1/50 dilution and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021), expandable thumbnail

    Western blot - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021)

    This data was developed using Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359) at 1/50000 dilution

    All lanes: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 93 kDa

    Observed band size: 156 kDa

  • Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021), expandable thumbnail

    Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021)

    This data was developed using Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359, the same antibody clone in a different buffer formulation.Immunoprecipitation of Human fetal brain labeling Mint-1 with Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000

    All lanes: Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - N-terminal (Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359)

    Predicted band size: 93 kDa

  • Western blot - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021), expandable thumbnail

    Western blot - Anti-Mint-1 antibody [EPR15941] - BSA and Azide free (ab251021)

    This data was developed using Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (Anti-Mint-1 antibody [EPR15941] - N-terminal ab190359) at 1/10000 dilution

    All lanes: Human cerebellum tissue lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 93 kDa

    Observed band size: 156 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com