Anti-Mint-1 antibody [EPR15941] - N-terminal

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling Mint-1 with ab190359 at 1/500 (1.964 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody as secondary at 1/1000 (2 μg/ml) dilution. Confocal image showing positive staining in IMR-32 cells (shown in green). ab7291 Anti-alpha Tubulin mouse monoclonal antibody at 1/1000 (1μg/ml) was used as a counterstain along with ab150120 as secondary at 1/1000 (2μg/ml) (shown in magenta). Negative control : HeLa. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Mint-1 with ab190359 at 1/1000 dilution.

Low expression tissue : nearly no staining on human lung.

The section was incubated with ab190359 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human transitional cell carcinoma of the bladder labeling Mint-1 with ab190359 at 1/50 dilution and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Mint-1 with ab190359 at 1/1000 dilution.

Positive staining on human cerebrum tissue.

The section was incubated with ab190359 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

Immunoprecipitation of Human fetal brain labeling Mint-1 with ab190359 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000

All lanes:

Immunoprecipitation - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359)

Predicted band size: 93 kDa

false

• WB

Lab

Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Low expression : HeLa (PMID : 7678331).

All lanes:

Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human hippocampus tissue lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

IMR-32 (human neuroblastoma neuroblast ) whole cell lysate at 20 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 156 kDa,36 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

All lanes:

Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/10000 dilution

All lanes:

Human cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 93 kDa

Observed band size: 156 kDa

false

• WB

Supplier Data

Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (AB190359)

All lanes:

Western blot - Anti-Mint-1 antibody [EPR15941] - N-terminal (ab190359) at 1/50000 dilution

All lanes:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 93 kDa

Observed band size: 156 kDa

false