Rabbit Polyclonal MIOS antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MIOS aa 800 to C-terminus.
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Publications
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- Biological research 56:662023The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Chia Wu,Wei-Ting Wang,Ming-Chun Yang,Yu-Tsun Su,Jwu-Lai Yeh,Jong-Hau Hsu,Jiunn-Ren WuPubMed 38057829
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MIOS aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NXC5
Reactivity data
Select an application| WB | IP | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Cat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Sheep | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway (PubMed:23723238, PubMed:26586190, PubMed:27487210, PubMed:35831510, PubMed:36528027). The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex (PubMed:23723238, PubMed:26586190, PubMed:27487210, PubMed:35831510, PubMed:36528027). GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1 (PubMed:36528027). In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation (PubMed:36528027). In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex (PubMed:25263562, PubMed:25457612, PubMed:26586190, PubMed:27487210). Within the GATOR2 complex, MIOS is required to prevent autoubiquitination of WDR24, the catalytic subunit of the complex (PubMed:35831510). The GATOR2 complex is required for brain myelination (By similarity).
Alternative names
GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog, MIOS
Recommended products
Rabbit Polyclonal MIOS antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MIOS aa 800 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MIOS aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NXC5
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab202274 was affinity purified using an epitope specific to MIOS immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MIOS also known as mitotic spindle organizing protein or GATOR2 subunit is a protein that plays an important role in cell cycle progression. It has a mass of approximately 67 kDa. MIOS is expressed in various tissues with notable presence in those with high proliferative activity. This protein is primarily found in the cytoplasm and is associated with the mitotic spindle highlighting its specific involvement in cell division processes.
- Biological function summary
MIOS serves as a component of the GATOR2 complex which plays an important role in the regulation of the mTORC1 pathway a central pathway in cell growth and metabolism. As part of this complex MIOS regulates amino acid sensing and signal transduction necessary for cellular responses to nutrient availability. This ensures that cells grow and divide only in environments that support such activities.
- Pathways
MIOS interacts significantly within the mTOR signaling pathway. The GATOR2 complex where MIOS plays a part facilitates the regulation of mTORC1 by interacting with other proteins such as DEPDC5 and SESTRIN2. This pathway influences a variety of cellular processes including protein synthesis and autophagy. MIOS through its function in the complex affects the integration of growth signals and ultimately impacts cellular metabolism and homeostasis.
- Associated diseases and disorders
Disruptions in MIOS function connect to cancer and metabolic diseases given the involvement in the mTORC1 pathway. Aberrant MIOS expression or regulation may contribute to uncontrolled cell growth observed in some cancers where the mTOR pathway often shows hyperactivation. Additionally MIOS's role in nutrient sensing and metabolism links it to obesity and diabetes where the mTOR pathway's balance is disrupted. These conditions highlight MIOS's broader implications in disease when its pathways or interacting proteins such as GATOR2 components become dysregulated.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Immunoprecipitation - Anti-MIOS antibody (ab202274)
Detection of MIOS in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab202274 at 6 μg/mg lysate for IP (Lane 1). For WB detection an ab202274 was used at 0.4 μg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-MIOS antibody (ab202274)
Predicted band size: 99 kDa
Western blot - Anti-MIOS antibody (ab202274)
All lanes: Western blot - Anti-MIOS antibody (ab202274) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: TCMK-1 whole cell lysate at 50 µg
Lane 5: NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 3min
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