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AB202274

Anti-MIOS antibody

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(3 Publications)

Rabbit Polyclonal MIOS antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MIOS aa 800 to C-terminus.

View Alternative Names

GATOR2 complex protein MIOS, Missing oocyte meiosis regulator homolog, MIOS

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Immunoprecipitation - Anti-MIOS antibody (AB202274)
  • IP

Supplier Data

Immunoprecipitation - Anti-MIOS antibody (AB202274)

Detection of MIOS in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab202274 at 6 μg/mg lysate for IP (Lane 1). For WB detection an ab202274 was used at 0.4 μg/ml. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-MIOS antibody (ab202274)

Predicted band size: 99 kDa

false

Western blot - Anti-MIOS antibody (AB202274)
  • WB

Supplier Data

Western blot - Anti-MIOS antibody (AB202274)

All lanes:

Western blot - Anti-MIOS antibody (ab202274) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 99 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human MIOS aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NXC5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab202274 was affinity purified using an epitope specific to MIOS immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIOS also known as mitotic spindle organizing protein or GATOR2 subunit is a protein that plays an important role in cell cycle progression. It has a mass of approximately 67 kDa. MIOS is expressed in various tissues with notable presence in those with high proliferative activity. This protein is primarily found in the cytoplasm and is associated with the mitotic spindle highlighting its specific involvement in cell division processes.
Biological function summary

MIOS serves as a component of the GATOR2 complex which plays an important role in the regulation of the mTORC1 pathway a central pathway in cell growth and metabolism. As part of this complex MIOS regulates amino acid sensing and signal transduction necessary for cellular responses to nutrient availability. This ensures that cells grow and divide only in environments that support such activities.

Pathways

MIOS interacts significantly within the mTOR signaling pathway. The GATOR2 complex where MIOS plays a part facilitates the regulation of mTORC1 by interacting with other proteins such as DEPDC5 and SESTRIN2. This pathway influences a variety of cellular processes including protein synthesis and autophagy. MIOS through its function in the complex affects the integration of growth signals and ultimately impacts cellular metabolism and homeostasis.

Disruptions in MIOS function connect to cancer and metabolic diseases given the involvement in the mTORC1 pathway. Aberrant MIOS expression or regulation may contribute to uncontrolled cell growth observed in some cancers where the mTOR pathway often shows hyperactivation. Additionally MIOS's role in nutrient sensing and metabolism links it to obesity and diabetes where the mTOR pathway's balance is disrupted. These conditions highlight MIOS's broader implications in disease when its pathways or interacting proteins such as GATOR2 components become dysregulated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway (PubMed : 23723238, PubMed : 26586190, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex (PubMed : 23723238, PubMed : 26586190, PubMed : 27487210, PubMed : 35831510, PubMed : 36528027). GATOR2 probably acts as an E3 ubiquitin-protein ligase toward GATOR1 (PubMed : 36528027). In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation (PubMed : 36528027). In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex (PubMed : 25263562, PubMed : 25457612, PubMed : 26586190, PubMed : 27487210). Within the GATOR2 complex, MIOS is required to prevent autoubiquitination of WDR24, the catalytic subunit of the complex (PubMed : 35831510). The GATOR2 complex is required for brain myelination (By similarity).
See full target information MIOS
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:291 PubMed41013205

2025

YULINK deficiency promotes cell death under glucose restriction in HCC cells in association with GLUT1-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chia Wu,Tsai-Hsien Hung,Wei-Ting Thomas Wang,Ming-Wei Kuo,Yuh-Jin Liang,Yur-Ren Kuo,Ming-Feng Hou,Chung-Sheng Lai,Alice L Yu,John Yu

Scientific reports 13:22164 PubMed38092761

2023

Morphological and cytoskeleton changes in cells after EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Assel Nurmagambetova,Vadim Mustyatsa,Aleena Saidova,Ivan Vorobjev

Biological research 56:66 PubMed38057829

2023

The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chia Wu,Wei-Ting Wang,Ming-Chun Yang,Yu-Tsun Su,Jwu-Lai Yeh,Jong-Hau Hsu,Jiunn-Ren Wu
View all publications
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