AB154639

Anti-MIOX antibody

Name: Anti-MIOX antibody (ab154639) | Abcam
Brand: Abcam Limited
SKU: AB154639
Price: 545 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal MIOX antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIOX aa 1-250.

View Alternative Names

ALDRL6, KSP32, RSOR, MIOX, Inositol oxygenase, Aldehyde reductase-like 6, Kidney-specific protein 32, Myo-inositol oxygenase, Renal-specific oxidoreductase, MI oxygenase

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIOX antibody (AB154639)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIOX antibody (AB154639)

Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MIOX with ab154639 at a 1/500 dilution.

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Western blot - Anti-MIOX antibody (AB154639)

12% SDS PAGE

All lanes:

Western blot - Anti-MIOX antibody (ab154639) at 1/2000 dilution

All lanes:

mouse kidney whole cell lysate at 50 µg

Predicted band size: 33 kDa

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Western blot - Anti-MIOX antibody (AB154639)

10% SDS PAGE

All lanes:

Western blot - Anti-MIOX antibody (ab154639) at 1/2000 dilution

All lanes:

rat kidney whole cell lysate at 50 µg

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MIOX aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UGB7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MIOX also known as myo-inositol oxygenase plays an important role in the conversion of myo-inositol into D-glucuronic acid. This metabolic enzyme has a mass of about 33 kDa. It is expressed mainly in the kidney particularly in the proximal tubules. MIOX functions as a monomer and contains a non-heme iron at its active site which is essential for its enzymatic activity.

Biological function summary

MIOX is involved in myo-inositol metabolism an important process for maintaining cellular osmolyte balance and signaling. It does not form part of a larger protein complex. Myo-inositol metabolism impacts various cellular functions including phosphoinositide synthesis which is important for cellular signaling pathways. MIOX by controlling myo-inositol levels therefore influences these cellular processes that are critical for kidney function.

Pathways

MIOX is a part of the inositol phosphate metabolism pathway. This pathway is critical for glucose and lipid metabolism affecting energy balance and cellular homeostasis. Additionally MIOX interacts with proteins involved in glucose metabolism such as enzymes in the glycolytic pathway highlighting its role in energy production and regulation. These interactions showcase MIOX's integration in broader metabolic networks.

Abnormal MIOX activity links to conditions such as diabetic nephropathy and kidney injury. MIOX overexpression has been observed in diabetic nephropathy where it might contribute to oxidative stress and kidney damage. Additionally altered MIOX activity associates with proteins like SOD1 which is implicated in oxidative stress responses. Understanding MIOX's involvement offers potential therapeutic insights for managing related renal disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MIOX

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 20:224-233 PubMed38767487

2024

Small extracellular vesicles derived from cerebral endothelial cells with elevated microRNA 27a promote ischemic stroke recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Zhongwu Liu,Michael Chopp,Michael Millman,Yanfeng Li,Pasquale Cepparulo,Amy Kemper,Chao Li,Li Zhang,Zheng Gang Zhang

Immunity, inflammation and disease 11:e829 PubMed37249295

2023

Myo-inositol oxygenase (MIOX) accelerated inflammation in the model of infection-induced cardiac dysfunction by NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Zhou,Congyi Yu,Yiwen Long

BMC genomics 24:127 PubMed36932340

2023

Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Zhu,Deng Qiong,Gu Yanli,Li Min,Zhang Ying,Hu Qiyi,Zhang Shenping,Wang Xisheng,Liang Hui

Cell death and differentiation 29:1850-1863 PubMed35338333

2022

Long noncoding RNA NEAT1 promotes ferroptosis by modulating the miR-362-3p/MIOX axis as a ceRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Meiying Luo,Xiaohong Cui,Douglas O'Connell,Yongfei Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MIOX antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIOX antibody (AB154639)

Western blot - Anti-MIOX antibody (AB154639)

Western blot - Anti-MIOX antibody (AB154639)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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