Rabbit Polyclonal MIOX antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIOX aa 1-250.
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Publications
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- Immunity, inflammation and disease 11:e8292023Myo-inositol oxygenase (MIOX) accelerated inflammation in the model of infection-induced cardiac dysfunction by NLRP3 inflammasome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjun Zhou,Congyi Yu,Yiwen LongPubMed 37249295
- Cell death and differentiation 29:1850-18632022Long noncoding RNA NEAT1 promotes ferroptosis by modulating the miR-362-3p/MIOX axis as a ceRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Zhang,Meiying Luo,Xiaohong Cui,Douglas O'Connell,Yongfei YangPubMed 35338333
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MIOX aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UGB7
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Alternative names
ALDRL6, KSP32, RSOR, MIOX, Inositol oxygenase, Aldehyde reductase-like 6, Kidney-specific protein 32, Myo-inositol oxygenase, Renal-specific oxidoreductase, MI oxygenase
Recommended products
Rabbit Polyclonal MIOX antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIOX aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MIOX aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UGB7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein MIOX also known as myo-inositol oxygenase plays an important role in the conversion of myo-inositol into D-glucuronic acid. This metabolic enzyme has a mass of about 33 kDa. It is expressed mainly in the kidney particularly in the proximal tubules. MIOX functions as a monomer and contains a non-heme iron at its active site which is essential for its enzymatic activity.
- Biological function summary
MIOX is involved in myo-inositol metabolism an important process for maintaining cellular osmolyte balance and signaling. It does not form part of a larger protein complex. Myo-inositol metabolism impacts various cellular functions including phosphoinositide synthesis which is important for cellular signaling pathways. MIOX by controlling myo-inositol levels therefore influences these cellular processes that are critical for kidney function.
- Pathways
MIOX is a part of the inositol phosphate metabolism pathway. This pathway is critical for glucose and lipid metabolism affecting energy balance and cellular homeostasis. Additionally MIOX interacts with proteins involved in glucose metabolism such as enzymes in the glycolytic pathway highlighting its role in energy production and regulation. These interactions showcase MIOX's integration in broader metabolic networks.
- Associated diseases and disorders
Abnormal MIOX activity links to conditions such as diabetic nephropathy and kidney injury. MIOX overexpression has been observed in diabetic nephropathy where it might contribute to oxidative stress and kidney damage. Additionally altered MIOX activity associates with proteins like SOD1 which is implicated in oxidative stress responses. Understanding MIOX's involvement offers potential therapeutic insights for managing related renal disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-MIOX antibody (ab154639)
10% SDS PAGE
All lanes: Western blot - Anti-MIOX antibody (ab154639) at 1/2000 dilution
All lanes: rat kidney whole cell lysate at 50 µg
Predicted band size: 33 kDa
Western blot - Anti-MIOX antibody (ab154639)
12% SDS PAGE
All lanes: Western blot - Anti-MIOX antibody (ab154639) at 1/2000 dilution
All lanes: mouse kidney whole cell lysate at 50 µg
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIOX antibody (ab154639)
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling MIOX with ab154639 at a 1/500 dilution.
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