AB251291

Anti-MIOX antibody [EPR17172] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MIOX antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples.

View Alternative Names

ALDRL6, KSP32, RSOR, MIOX, Inositol oxygenase, Aldehyde reductase-like 6, Kidney-specific protein 32, Myo-inositol oxygenase, Renal-specific oxidoreductase, MI oxygenase

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Supplier Data

Immunoprecipitation - Anti-MIOX antibody [EPR17172] - BSA and Azide free (AB251291)

This data was developed using ab199729, the same antibody clone in a different buffer formulation.

MIOX was immunoprecipitated from 1mg of Mouse kidney whole cell lysate with ab199729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199729 at 1/2000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Mouse kidney whole cell lysate 10 μg (Input). Lane 2 : ab199729 IP in Mouse kidney whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199729 in Mouse kidney whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MIOX antibody [EPR17172] (<a href='/en-us/products/primary-antibodies/miox-antibody-epr17172-ab199729'>ab199729</a>)

Predicted band size: 33 kDa

false

Lab

Western blot - Anti-MIOX antibody [EPR17172] - BSA and Azide free (AB251291)

This data was developed using ab199729, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human fetal heart, Human fetal spleen and HeLa don't express MIOX.

All lanes:

Western blot - Anti-MIOX antibody [EPR17172] (<a href='/en-us/products/primary-antibodies/miox-antibody-epr17172-ab199729'>ab199729</a>) at 1/5000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal spleen tissue lysate at 10 µg

Lane 4:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 10s

Lab

Western blot - Anti-MIOX antibody [EPR17172] - BSA and Azide free (AB251291)

This data was developed using ab199729, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Mouse heart, mouse brain, mouse spleen, RAW264.7, and NIH/3T3 don't express MIOX.

All lanes:

Western blot - Anti-MIOX antibody [EPR17172] (<a href='/en-us/products/primary-antibodies/miox-antibody-epr17172-ab199729'>ab199729</a>) at 1/5000 dilution

Lane 1:

Mouse kidney tissue lysate at 10 µg

Lane 2:

Mouse brain tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 6:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

false

Exposure time: 10s

Unconjugated

Anti-MIOX antibody [EPR17172]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17172

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab251291 is the carrier-free version of ab199729.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MIOX also known as myo-inositol oxygenase plays an important role in the conversion of myo-inositol into D-glucuronic acid. This metabolic enzyme has a mass of about 33 kDa. It is expressed mainly in the kidney particularly in the proximal tubules. MIOX functions as a monomer and contains a non-heme iron at its active site which is essential for its enzymatic activity.

Biological function summary

MIOX is involved in myo-inositol metabolism an important process for maintaining cellular osmolyte balance and signaling. It does not form part of a larger protein complex. Myo-inositol metabolism impacts various cellular functions including phosphoinositide synthesis which is important for cellular signaling pathways. MIOX by controlling myo-inositol levels therefore influences these cellular processes that are critical for kidney function.

Pathways

MIOX is a part of the inositol phosphate metabolism pathway. This pathway is critical for glucose and lipid metabolism affecting energy balance and cellular homeostasis. Additionally MIOX interacts with proteins involved in glucose metabolism such as enzymes in the glycolytic pathway highlighting its role in energy production and regulation. These interactions showcase MIOX's integration in broader metabolic networks.

Abnormal MIOX activity links to conditions such as diabetic nephropathy and kidney injury. MIOX overexpression has been observed in diabetic nephropathy where it might contribute to oxidative stress and kidney damage. Additionally altered MIOX activity associates with proteins like SOD1 which is implicated in oxidative stress responses. Understanding MIOX's involvement offers potential therapeutic insights for managing related renal disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MIOX

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MIOX antibody [EPR17172] - BSA and Azide free

Immunoprecipitation - Anti-MIOX antibody [EPR17172] - BSA and Azide free (AB251291)

Western blot - Anti-MIOX antibody [EPR17172] - BSA and Azide free (AB251291)

Western blot - Anti-MIOX antibody [EPR17172] - BSA and Azide free (AB251291)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise