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AB245831

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free

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Rabbit Recombinant Monoclonal MIP-1 alpha/CCL3 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

G0S19-1, MIP1A, SCYA3, CCL3, C-C motif chemokine 3, G0/G1 switch regulatory protein 19-1, Macrophage inflammatory protein 1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein, MIP-1-alpha

4 Images
Flow Cytometry (Intracellular) - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)

This data was developed using ab206429, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized THP-1 (human monocytic leukemia cell line) (treated with 80nM TPA overnight, then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3 hr) (red) or untreated (green) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab206429 at 1/600 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)

This data was developed using ab206429, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab206429 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in THP-1 cells treated with TPA (80nM overnight), then treated with 100ng/ml LPS for 3h and together with 300ng/ml BFA for another 3h. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)
  • WB

Unknown

Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)

This data was developed using ab206429, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] (<a href='/en-us/products/primary-antibodies/mip-1-alpha-ccl3-ccl4-mip-1-beta-ccl3l1-ccl4l1-antibody-epr19900-275-ab206429'>ab206429</a>) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 10 µg

Lane 2:

THP-1 (1 treated with 80nM TPA overnight, then 100 ng/ml LPS for 3 hours and then 300 ng/ml Brefeldin A was added for the last 3 hours) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa

false

Exposure time: 3min

Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)
  • WB

Lab

Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] - BSA & Azide free (AB245831)

Exposure time :
Lanes 1-7 (left image) : 180 seconds
Lane 6 (right image) : 5 seconds

This data was developed using ab206429, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275] (<a href='/en-us/products/primary-antibodies/mip-1-alpha-ccl3-ccl4-mip-1-beta-ccl3l1-ccl4l1-antibody-epr19900-275-ab206429'>ab206429</a>) at 1/1000 dilution

Lane 1:

His-tagged mouse CCL3 Recombinant Protein

Lane 2:

His-tagged human CCL3 Recombinant Protein

Lane 3:

His-tagged human CCL4 Recombinant Protein

Lanes 4 - 5:

His-tagged human CCL18 Recombinant Protein

Lane 6:

GST-tagged human CCL3L1 Recombinant Protein

Lane 7:

GST-tagged human CCL4L1 Recombinant Protein

false

  • Unconjugated

    Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta + CCL3L1 + CCL4L1 antibody [EPR19900-275]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19900-275

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab245831 is the carrier-free version of ab206429.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-1 alpha also known as CCL3 and MIP-1 beta known as CCL4 are key chemokines involved in the immune response. These proteins have a molecular mass of approximately 8 to 10 kDa. They are secreted by various cells including macrophages dendritic cells and lymphocytes. MIP-1 alpha and MIP-1 beta are part of the CC chemokine family and are secreted as monomers that bind to specific receptors on the surface of target cells attracting and activating them during inflammation and immune responses.
Biological function summary

These chemokines play significant roles in the recruitment and activation of leukocytes to sites of infection or injury. They are important elements in the immune defense system and help to orchestrate the movement and coordination of immune cells. MIP-1 alpha and MIP-1 beta function in synergy with other chemokines and cytokines enhancing the body's ability to respond to pathogens. While they do not form part of a larger complex their interaction with chemokine receptors like CCR1 CCR4 and CCR5 is critical for their function.

Pathways

MIP-1 alpha and MIP-1 beta participate in inflammatory and immune pathways. They are key mediators in the chemokine signaling pathway linked especially with the activation of the MAPK and NF-kB pathways. These chemokines work closely with related chemokines such as CCL5 (RANTES) and cytokines like TNF-alpha amplifying the body's response to immune challenges.

MIP-1 alpha and MIP-1 beta have connections to several pathological conditions particularly inflammatory diseases and HIV. Their levels often increase in diseases like rheumatoid arthritis reflecting their role in inflammatory processes. In the context of HIV infection these chemokines inhibit viral entry by blocking the CCR5 receptor which is important for some strains of the virus. This highlights their therapeutic potential in designing treatments targeting HIV alongside other inflammatory conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
See full target information CCL3

Additional targets

CCL4,CCL4L1,CCL3L1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com