JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259471

Anti-MIP-3 beta/CCL19 antibody [EPR21931-74] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MIP-3 beta/CCL19 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Elc, Scya19, Ccl19, C-C motif chemokine 19, Epstein-Barr virus-induced molecule 1 ligand chemokine, Small-inducible cytokine A19, EBI1 ligand chemokine, ELC

1 Images
Sandwich ELISA - Anti-MIP-3 beta/CCL19 antibody [EPR21931-74] - BSA and Azide free (Capture) (AB259471)
  • sELISA

Unknown

Sandwich ELISA - Anti-MIP-3 beta/CCL19 antibody [EPR21931-74] - BSA and Azide free (Capture) (AB259471)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab242236).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21931-74

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab259471 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse CCL19. The recommended pair for sandwich ELISA is:
Capture: ab259471, Mouse CCL19 Capture Antibody (unconjugated)
Detector: ab259472, Mouse CCL19 Detector Antibody (unconjugated)
The reference range value is 4.69 - 300 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIP-3 beta also known as CCL19 is a chemokine involved in immune responses. It weighs around 11 kDa and is encoded by the CCL19 gene. MIP-3 beta does its function by binding to its receptor CCR7 which is common in lymph nodes and other lymphoid tissues. It recruits and directs the migration of immune cells especially T cells and dendritic cells navigating them towards lymphoid tissues. This makes MIP-3 beta integral in initiating and modulating immune responses.
Biological function summary

MIP-3 beta helps direct cell trafficking within the lymphoid organs. It is not part of a complex but its interaction with the CCR7 receptor is essential for its biological activity. This binding plays a role in the development of secondary lymphoid organs and immune surveillance. Furthermore MIP-3 beta supports the homing of immune cells to appropriate locations impacting both innate and adaptive immune responses.

Pathways

MIP-3 beta is associated with the chemokine signaling pathway and the immune system pathway. Through these pathways it interacts with proteins like CCR7 and other chemokines that regulate leukocyte migration and activation. These pathways ensure local and systemic immune responses are efficiently orchestrated impacting inflammation and immune regulation.

MIP-3 beta has connections to autoimmune diseases like rheumatoid arthritis and cancer. Its deregulation can result in abnormal immune surveillance contributing to the pathology of these conditions. MIP-3 beta regulates immune cell infiltration and any imbalance may also link it to other inflammatory disorders. Relations to proteins such as CCR7 in these conditions emphasize its role in disease pathogenesis and highlight its potential as a therapeutic target.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information Ccl19
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB245723

Human CCL19 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CCL19 ELISA Kit (AB245723)

  • 0
  • 0
Primary Antibodies

AB242720

Anti-Eotaxin 2 antibody [EPR17018-243] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Eotaxin 2 antibody [EPR17018-243] - BSA and Azide free (Detector) (AB242720)

  • 0
  • 0
Primary Antibodies

AB124768

Anti-MDC antibody [EPR1362]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC antibody [EPR1362] (AB124768)

  • 0
  • 0
Primary Antibodies

AB242558

Anti-TARC/CCL17 antibody [EPR17866-42] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-TARC/CCL17 antibody [EPR17866-42] - BSA and Azide free (Capture) (AB242558)

  • 0
  • 0
Primary Antibodies

AB242714

Anti-CXCL11 antibody [EPR17809-107] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CXCL11 antibody [EPR17809-107] - BSA and Azide free (Detector) (AB242714)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com