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Rabbit Recombinant Monoclonal MIP-3 beta/CCL19 antibody. Suitable for IHC-P, WB and reacts with Transfected cell line - Mouse, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (AB324163), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (AB324163), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (AB324163), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (AB324163), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (AB324163), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow CytICC/IF
Mouse
Tested
Tested
Not recommended
Not recommended
Transfected cell line - Mouse
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Mouse
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell line - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell line - Mouse
Dilution info
-
Notes

-

Target data

Function

Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Alternative names

Elc, Scya19, Ccl19, C-C motif chemokine 19, Epstein-Barr virus-induced molecule 1 ligand chemokine, Small-inducible cytokine A19, EBI1 ligand chemokine, ELC

Rabbit Recombinant Monoclonal MIP-3 beta/CCL19 antibody. Suitable for IHC-P, WB and reacts with Transfected cell line - Mouse, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27463-29
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163)

    MIP-3 beta/CCL19 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-MIP-3 beta/CCL19 antibody

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse CCL19 expression vector containing a His tag. (B) HEK-293T transfected with a Human CCL19 expression vector containing a His tag. (C) HEK-293T transfected with empty vector containing a His tag. tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on HEK-293T transfected with a His-tagged Mouse CCL19 construct (image A), no staining on HEK-293T transfected with a His-tagged Human CCL19 construct (image B) and HEK-293T transfected with empty vector containing a His tag (image C) . The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163)

    MIP-3 beta/CCL19 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-MIP-3 beta/CCL19 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: no staining on mouse liver. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163)

    MIP-3 beta/CCL19 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse lymph node using rabbit Anti-MIP-3 beta/CCL19 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse lymph node tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse lymph node. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163)

    MIP-3 beta/CCL19 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse thymus using rabbit Anti-MIP-3 beta/CCL19 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse thymus. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163)

    MIP-3 beta/CCL19 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse spleen using rabbit Anti-MIP-3 beta/CCL19 antibody

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163), expandable thumbnail

    Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163)

    MIP-3 beta/CCL19 Western blot staining of Mouse lymph node tissue lysate using rabbit Anti-MIP-3 beta/CCL19 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the bands higher than 12kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163) at 1/1000 dilution

    All lanes: Mouse lymph node tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 12 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163), expandable thumbnail

    Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163)

    MIP-3 beta/CCL19 Western blot staining using rabbit Anti-MIP-3 beta/CCL19 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: brain (PMID:9653094)

    The identity of the bands higher than 12kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (ab324163) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 40 µg

    Lane 2: Mouse brain tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 12 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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