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AB154407

Anti-MIPEP antibody

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(1 Publication)

Rabbit Polyclonal MIPEP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MIPEP aa 150-400.

View Alternative Names

MIP, MIPEP, Mitochondrial intermediate peptidase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MIPEP antibody (AB154407)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MIPEP antibody (AB154407)

Immunofluorescence analysis of methanol-fixed A431 cells, labeling MIPEP using ab154407 at a 1/200 dilution.
The image in the lower panel was co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIPEP antibody (AB154407)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIPEP antibody (AB154407)

Immunohistochemical analysis of paraffin embedded Human CL15 xenograft tissue, labeling MIPEP using ab154407 at a 1/100 dilution.

Western blot - Anti-MIPEP antibody (AB154407)
  • WB

Unknown

Western blot - Anti-MIPEP antibody (AB154407)

7.5% SDS PAGE

All lanes:

Western blot - Anti-MIPEP antibody (ab154407) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 81 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MIPEP aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q99797

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIPEP also known as mitochondrial intermediate peptidase is a mitochondrial enzyme with a mass of approximately 78 kDa. This protein functions in the mitochondria where it processes precursor proteins by cleaving specific N-terminal residues after import into the organelle. MIPEP is expressed in a wide range of tissues but shows higher levels in the heart skeletal muscle and kidney. Its enzymatic activity is important for the maturation of mitochondrial proteins which are needed for proper mitochondrial function.
Biological function summary

MIPEP plays an important role in the processing and maturation of several mitochondrial matrix proteins. It acts in concert with other processing peptidases assisting in the final steps of protein maturation after the mitochondrial processing peptidase (MPP) cleaves the leader sequences from proteins. MIPEP works as part of a proteolytic system within the mitochondria that ensures proteins are in their functional forms. This processing ability is integral to maintaining the integrity and efficiency of the mitochondrial respiratory chain.

Pathways

MIPEP contributes significantly to mitochondrial protein import and processing pathways. The protein is involved in the maturation pathway of mitochondrial proteins which is critical for assembling electron transport chain complexes. MIPEP interacts with related peptidases such as MPP facilitating the process of protein maturation needed for efficient mitochondrial energy production. This interaction is essential for linking protein processing with energy metabolism.

Abnormalities in MIPEP function have been associated with combined oxidative phosphorylation deficiency. This disorder impairs energy production leading to a range of symptoms such as muscle weakness and neurological deficits. Additionally MIPEP dysfunction has links to mitochondrial diseases where it might affect proteins like MPP disrupting normal protein processing and exacerbating disease phenotypes. Understanding MIPEP's role may provide insights into the molecular basis of mitochondrial disorders and aid in developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves proteins, imported into the mitochondrion, to their mature size.
See full target information MIPEP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 33:145-159.e6 PubMed33357456

2020

High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sangmoo Jeong,Angela Maria Savino,Rachel Chirayil,Ersilia Barin,Yuanming Cheng,Sun-Mi Park,Alexandra Schurer,Edouard Mullarky,Lewis C Cantley,Michael G Kharas,Kayvan R Keshari
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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