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Rabbit Polyclonal MIPEP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MIPEP aa 150-400.

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Images

Western blot - Anti-MIPEP antibody (AB154407), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MIPEP antibody (AB154407), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIPEP antibody (AB154407), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human MIPEP aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99797

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Target data

Function

Cleaves proteins, imported into the mitochondrion, to their mature size.

Alternative names

Recommended products

Rabbit Polyclonal MIPEP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MIPEP aa 150-400.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human MIPEP aa 150-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99797
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MIPEP also known as mitochondrial intermediate peptidase is a mitochondrial enzyme with a mass of approximately 78 kDa. This protein functions in the mitochondria where it processes precursor proteins by cleaving specific N-terminal residues after import into the organelle. MIPEP is expressed in a wide range of tissues but shows higher levels in the heart skeletal muscle and kidney. Its enzymatic activity is important for the maturation of mitochondrial proteins which are needed for proper mitochondrial function.

Biological function summary

MIPEP plays an important role in the processing and maturation of several mitochondrial matrix proteins. It acts in concert with other processing peptidases assisting in the final steps of protein maturation after the mitochondrial processing peptidase (MPP) cleaves the leader sequences from proteins. MIPEP works as part of a proteolytic system within the mitochondria that ensures proteins are in their functional forms. This processing ability is integral to maintaining the integrity and efficiency of the mitochondrial respiratory chain.

Pathways

MIPEP contributes significantly to mitochondrial protein import and processing pathways. The protein is involved in the maturation pathway of mitochondrial proteins which is critical for assembling electron transport chain complexes. MIPEP interacts with related peptidases such as MPP facilitating the process of protein maturation needed for efficient mitochondrial energy production. This interaction is essential for linking protein processing with energy metabolism.

Associated diseases and disorders

Abnormalities in MIPEP function have been associated with combined oxidative phosphorylation deficiency. This disorder impairs energy production leading to a range of symptoms such as muscle weakness and neurological deficits. Additionally MIPEP dysfunction has links to mitochondrial diseases where it might affect proteins like MPP disrupting normal protein processing and exacerbating disease phenotypes. Understanding MIPEP's role may provide insights into the molecular basis of mitochondrial disorders and aid in developing therapeutic strategies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-MIPEP antibody (ab154407), expandable thumbnail

    Western blot - Anti-MIPEP antibody (ab154407)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-MIPEP antibody (ab154407) at 1/1000 dilution

    All lanes: A431 whole cell lysate at 30 µg

    Predicted band size: 81 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MIPEP antibody (ab154407), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MIPEP antibody (ab154407)

    Immunofluorescence analysis of methanol-fixed A431 cells, labeling MIPEP using ab154407 at a 1/200 dilution.
    The image in the lower panel was co-stained with Hoechst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIPEP antibody (ab154407), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIPEP antibody (ab154407)

    Immunohistochemical analysis of paraffin embedded Human CL15 xenograft tissue, labeling MIPEP using ab154407 at a 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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