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AB197788

Anti-Miranda antibody [CD#5- 7E9BG5AF4]

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(19 Publications)

Rat Monoclonal Miranda antibody. Suitable for IHC-P and reacts with Drosophila melanogaster samples. Cited in 19 publications.

View Alternative Names

CG12249, Dmel\CG12249, Mir

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Miranda antibody [CD#5- 7E9BG5AF4] (AB197788)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Miranda antibody [CD#5- 7E9BG5AF4] (AB197788)

Immunohistochemistry analysis using Miranda rat monoclonal to detect Miranda protein in all neuroblasts from Drosophila stage 10 embryos. Lateral view showing asymmetric partitioning of Miranda protein to the basal cortex of the neuroblasts, and then specifically into the smaller basal daughter cells (GMCs). The Miranda antibody was used at 3.4 μg/mL and detected with Alexa488 conjugated goat anti-rat at 1 : 400. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

CD#5- 7E9BG5AF4

Isotype

IgG2b

Carrier free

No

Reacts with

Drosophila melanogaster

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification notes
Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Miranda
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39255019

2024

Glial ferritin maintains neural stem cells via transporting iron required for self-renewal in .

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Ma,Wenshu Wang,Xiaojing Yang,Menglong Rui,Su Wang

Experimental & molecular medicine 56:422-440 PubMed38374207

2024

Baf-mediated transcriptional regulation of teashirt is essential for the development of neural progenitor cell lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Byung Su Ko,Myeong Hoon Han,Min Jee Kwon,Dong Gon Cha,Yuri Ji,Eun Seo Park,Min Jae Jeon,Somi Kim,Kyeongho Lee,Yoon Ha Choi,Jusung Lee,Monica Torras-Llort,Ki-Jun Yoon,Hyosang Lee,Jong Kyoung Kim,Sung Bae Lee

iScience 27:108545 PubMed38213621

2024

Golgi-to-ER retrograde transport prevents premature differentiation of type II neuroblasts via Notch-signal-sending daughter cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Zhang,Menglong Rui,Zhixin Ma,Sifan Gong,Shuliu Zhang,Qingxia Zhou,Congfeng Gan,Wenting Gong,Su Wang

The Journal of cell biology 222: PubMed37589718

2023

Cooperative regulation of C1-domain membrane recruitment polarizes atypical protein kinase C.

Applications

Unspecified application

Species

Unspecified reactive species

Kimberly A Jones,Michael L Drummond,Rhiannon R Penkert,Kenneth E Prehoda

EMBO reports 23:e53813 PubMed35993198

2022

Lint-O cooperates with L(3)mbt in target gene suppression to maintain homeostasis in fly ovary and brain.

Applications

Unspecified application

Species

Unspecified reactive species

Hitomi Yamamoto-Matsuda,Keita Miyoshi,Mai Moritoh,Hikari Yoshitane,Yoshitaka Fukada,Kuniaki Saito,Soichiro Yamanaka,Mikiko C Siomi

Disease models & mechanisms 15: PubMed35972154

2022

Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survival.

Applications

Unspecified application

Species

Unspecified reactive species

Kathrin Pfeifer,Georg Wolfstetter,Vimala Anthonydhason,Tafheem Masudi,Badrul Arefin,Mats Bemark,Patricia Mendoza-Garcia,Ruth H Palmer

Cell reports 37:109895 PubMed34706235

2021

Peripheral astral microtubules ensure asymmetric furrow positioning in neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Thomas,Emmanuel Gallaud,Aude Pascal,Laurence Serre,Isabelle Arnal,Laurent Richard-Parpaillon,Matthew Scott Savoian,Régis Giet

EMBO reports 22:e52130 PubMed33751817

2021

Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Dong,Michael Zavortink,Francesca Froldi,Sofya Golenkina,Tammy Lam,Louise Y Cheng

PLoS genetics 16:e1008913 PubMed33211700

2020

Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rohan Chippalkatti,Boris Egger,Beat Suter

Glia 68:2503-2516 PubMed32531091

2020

Overexpression of H3K36 methyltransferase NSD in glial cells affects brain development in Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Taejoon Kim,Hyewon Shin,Bokyeong Song,Chihyun Won,Hideki Yoshida,Masamitsu Yamaguchi,Kyoung Sang Cho,Im-Soon Lee
View all publications
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