Rat Monoclonal Miranda antibody. Suitable for IHC-P and reacts with Drosophila melanogaster samples. Cited in 14 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Miranda antibody [CD#5- 7E9BG5AF4] (AB197788), expandable thumbnail](https://content.abcam.com/products/images/miranda-antibody-cd5-7e9bg5af4-ab197788--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110392.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Miranda antibody [CD#5- 7E9BG5AF4] (AB197788)")
Publications
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- EMBO reports 23:e538132022Lint-O cooperates with L(3)mbt in target gene suppression to maintain homeostasis in fly ovary and brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHitomi Yamamoto-Matsuda,Keita Miyoshi,Mai Moritoh,Hikari Yoshitane,Yoshitaka Fukada,Kuniaki Saito,Soichiro Yamanaka,Mikiko C SiomiPubMed 35993198
- Disease models & mechanisms 15:2022Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathrin Pfeifer,Georg Wolfstetter,Vimala Anthonydhason,Tafheem Masudi,Badrul Arefin,Mats Bemark,Patricia Mendoza-Garcia,Ruth H PalmerPubMed 35972154
- Cell reports 37:1098952021Peripheral astral microtubules ensure asymmetric furrow positioning in neural stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandre Thomas,Emmanuel Gallaud,Aude Pascal,Laurence Serre,Isabelle Arnal,Laurent Richard-Parpaillon,Matthew Scott Savoian,Régis GietPubMed 34706235
- EMBO reports 22:e521302021Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Dong,Michael Zavortink,Francesca Froldi,Sofya Golenkina,Tammy Lam,Louise Y ChengPubMed 33751817
- PLoS genetics 16:e10089132020Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRohan Chippalkatti et. al.PubMed 33211700
- Current biology : CB 30:893-898.e52020A Conserved PDZ-Binding Motif in aPKC Interacts with Par-3 and Mediates Cortical Polarity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan W Holly et. al.PubMed 32084408
- Developmental cell 52:277-293.e82019Mitotic Implantation of the Transcription Factor Prospero via Phase Separation Drives Terminal Neuronal Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaodan Liu,Jingwen Shen,Leiming Xie,Zelin Wei,Chouin Wong,Yiyao Li,Xinhe Zheng,Pilong Li,Yan SongPubMed 31866201
- Developmental cell 51:713-729.e62019Mutations in ANKLE2, a ZIKA Virus Target, Disrupt an Asymmetric Cell Division Pathway in Drosophila Neuroblasts to Cause Microcephaly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNichole Link et. al.PubMed 31735666
- eLife 7:2018The retromer complex safeguards against neural progenitor-derived tumorigenesis by regulating Notch receptor trafficking.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Li,Chouin Wong,Shihong Max Gao,Rulan Zhang,Rongbo Sun,Yulong Li,Yan SongPubMed 30176986
- eLife 7:2018A sleep state in larvae required for neural stem cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilan Szuperak et. al.PubMed 29424688
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Drosophila melanogaster | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info - | Notes - |
Target data
Alternative names
CG12249, Dmel\CG12249, Mir
Recommended products
Rat Monoclonal Miranda antibody. Suitable for IHC-P and reacts with Drosophila melanogaster samples. Cited in 14 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- CD#5- 7E9BG5AF4
- Concentration
- Purification notes
Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Miranda antibody [CD#5- 7E9BG5AF4] (ab197788), expandable thumbnail](https://content.abcam.com/products/images/miranda-antibody-cd5-7e9bg5af4-ab197788--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110392.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Miranda antibody [CD#5- 7E9BG5AF4] (ab197788)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Miranda antibody [CD#5- 7E9BG5AF4] (ab197788)
Immunohistochemistry analysis using Miranda rat monoclonal to detect Miranda protein in all neuroblasts from Drosophila stage 10 embryos. Lateral view showing asymmetric partitioning of Miranda protein to the basal cortex of the neuroblasts, and then specifically into the smaller basal daughter cells (GMCs). The Miranda antibody was used at 3.4 μg/mL and detected with Alexa488 conjugated goat anti-rat at 1:400. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.
Downloads
Product protocols
- Visit the general protocols
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