Name: Anti-MIRO1 antibody [CL1083]
SKU: ab188029
Rating: 4 (4 reviews)

Anti-MIRO1 antibody [CL1083] (ab188029) is a mouse monoclonal antibody that is used to detect MIRO1 in Western Blot, IHC-P. Suitable for Human samples.

- Specificity confirmed with MIRO1 knockout cell line validation

Images

Western blot - Anti-MIRO1 antibody [CL1083] (AB188029), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Fragment Protein within Human RHOT1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXI2

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB
Human	Tested	Tested
Mouse	Predicted	Predicted
Rat	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500.00000 - 1/1000.00000	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information RHOT1

Function

Mitochondrial GTPase involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657). Promotes mitochondrial fission during high calcium conditions (PubMed:27716788).

Alternative names

ARHT1, RHOT1, Mitochondrial Rho GTPase 1, MIRO-1, hMiro-1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Fragment Protein within Human RHOT1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXI2
Clone number: CL1083
Purification technique: Affinity purification Immunogen
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-MIRO1 antibody [CL1083] (ab188029) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of MIRO1?

Anti-MIRO1 [CL1083] (ab188029) specifically detects a band for MIRO1 (UniProt: Q8IXI2) at a molecular weight of 71kDa.

Specificity confirmed

The specificity of Anti-MIRO1 antibody [CL1083] (ab188029) has been confirmed by Western blot testing in RHOT1 Knockout HeLa cell line, ab265792.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MIRO1 also known as RhoT1 is a mitochondrial Rho GTPase with a molecular mass of approximately 69 kDa. It functions as a pivotal component in mitochondrial transport and dynamics. This protein localizes to the outer mitochondrial membrane and is highly expressed in various tissues particularly in the brain and muscles. MIRO1 facilitates the anchoring of mitochondria to microtubule motors enabling their movement within the cell which is essential for maintaining proper energy distribution.

Biological function summary

MIRO1 plays a significant role in regulating mitochondrial trafficking along the cytoskeleton. It forms part of a protein complex with TRAK1/2 kinesin and dynein motor proteins. MIRO1's presence in this complex allows it to modulate mitochondrial distribution and dynamics which are essential for neural development and function. It also influences mitochondrial shape and interaction with the endoplasmic reticulum impacting intracellular calcium homeostasis.

Pathways

MIRO1 is an integral component of pathways involving mitochondrial transport and cellular energy homeostasis. It actively participates in the regulation of calcium signaling pathways alongside related proteins like TRAK1. Additionally MIRO1's involvement in the maintenance of mitochondrial dynamics links it to mitophagy pathways. These pathways regulate the removal of damaged mitochondria a vital process for cellular health and longevity.

Associated diseases and disorders

MIRO1 has connections to neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. These conditions correlate with impaired mitochondrial trafficking and dynamics. MIRO1 interacts with proteins such as PINK1 and Parkin which contribute to mitochondrial quality control and are involved in the pathogenesis of these diseases. The disruption of MIRO1 function can lead to mitochondrial dysfunction and energy deficits which are underlying factors in these neurodegenerative processes.

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9 product images

Western blot - Anti-MIRO1 antibody [CL1083] (ab188029)
False colour image of Western blot: Anti-MIRO1 antibody [CL1083] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab188029 was shown to bind specifically to MIRO1. A band was observed at 71 kDa in wild-type HeLa cell lysates with no signal observed at this size in RHOT1 knockout cell line Human RHOT1 (MIRO1) knockout HeLa cell line ab265792 (knockout cell lysate Human RHOT1 (MIRO1) knockout HeLa cell lysate ab258635). To generate this image, wild-type and RHOT1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
All lanes: Western blot - Anti-MIRO1 antibody [CL1083] (ab188029) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 40 µg
Lane 2: RHOT1 knockout HeLa cell lysate at 40 µg
Lane 2: Western blot - Human RHOT1 (MIRO1) knockout HeLa cell line (Human RHOT1 (MIRO1) knockout HeLa cell line ab265792)
Lane 3: HEK-293 cell lysate at 20 µg
Lane 4: A431 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Western blot - Anti-MIRO1 antibody [CL1083] (ab188029)
All lanes: Western blot - Anti-MIRO1 antibody [CL1083] (ab188029) at 1/500 dilution
All lanes: Human cerebral cortex tissue
Predicted band size: 71 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (ab188029)
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (ab188029)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (ab188029)
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (ab188029)
Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (ab188029)
Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (ab188029)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (ab188029)
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.