Anti-MIRO1 antibody [CL1083]

• WB

Lab

Western blot - Anti-MIRO1 antibody [CL1083] (AB188029)

False colour image of Western blot : Anti-MIRO1 antibody [CL1083] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab188029 was shown to bind specifically to MIRO1. A band was observed at 71 kDa in wild-type HeLa cell lysates with no signal observed at this size in RHOT1 knockout cell line ab265792 (knockout cell lysate ab258635). To generate this image, wild-type and RHOT1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-MIRO1 antibody [CL1083] (ab188029) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 40 µg

Lane 2:

RHOT1 knockout HeLa cell lysate at 40 µg

Lane 2:

Western blot - Human RHOT1 (MIRO1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rhot1-miro1-knockout-hela-cell-line-ab265792'>ab265792</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Predicted band size: 71 kDa

Observed band size: 71 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (AB188029)

Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (AB188029)

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (AB188029)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (AB188029)

Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (AB188029)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (AB188029)

Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO1 antibody [CL1083] (AB188029)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue, labeling MIRO1 using ab188029 at a 1/200 dilution.

• WB

Supplier Data

Western blot - Anti-MIRO1 antibody [CL1083] (AB188029)

All lanes:

Western blot - Anti-MIRO1 antibody [CL1083] (ab188029) at 1/500 dilution

All lanes:

Human cerebral cortex tissue

Predicted band size: 71 kDa

false