Knockout Tested Rabbit Recombinant Monoclonal MIRO1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Images
![Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (AB320018), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-16-bsa-and-azide-free-capture-ab320018--sandwich-elisa-img433963.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (AB320018)")
![Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (AB320018), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-16-bsa-and-azide-free-capture-ab320018--sandwich-elisa-img433964.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (AB320018)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Mitochondrial GTPase involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657). Promotes mitochondrial fission during high calcium conditions (PubMed:27716788).
Alternative names
ARHT1, RHOT1, Mitochondrial Rho GTPase 1, MIRO-1, hMiro-1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal MIRO1 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- MJF-D29136-16
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not cross-react with human MIRO2.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab320018 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify MIRO1. The recommended pair for sandwich ELISA is:
Capture: ab320018, MIRO1 Capture Antibody (unconjugated)
Detector: Anti-MIRO1 antibody [MJF-D29136-145] - BSA and Azide free (Detector) ab320019, MIRO1 Detector Antibody (unconjugated)
The reference range value is 0.31-20 ng/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MIRO1 also known as RhoT1 is a mitochondrial Rho GTPase with a molecular mass of approximately 69 kDa. It functions as a pivotal component in mitochondrial transport and dynamics. This protein localizes to the outer mitochondrial membrane and is highly expressed in various tissues particularly in the brain and muscles. MIRO1 facilitates the anchoring of mitochondria to microtubule motors enabling their movement within the cell which is essential for maintaining proper energy distribution.
- Biological function summary
MIRO1 plays a significant role in regulating mitochondrial trafficking along the cytoskeleton. It forms part of a protein complex with TRAK1/2 kinesin and dynein motor proteins. MIRO1's presence in this complex allows it to modulate mitochondrial distribution and dynamics which are essential for neural development and function. It also influences mitochondrial shape and interaction with the endoplasmic reticulum impacting intracellular calcium homeostasis.
- Pathways
MIRO1 is an integral component of pathways involving mitochondrial transport and cellular energy homeostasis. It actively participates in the regulation of calcium signaling pathways alongside related proteins like TRAK1. Additionally MIRO1's involvement in the maintenance of mitochondrial dynamics links it to mitophagy pathways. These pathways regulate the removal of damaged mitochondria a vital process for cellular health and longevity.
- Associated diseases and disorders
MIRO1 has connections to neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. These conditions correlate with impaired mitochondrial trafficking and dynamics. MIRO1 interacts with proteins such as PINK1 and Parkin which contribute to mitochondrial quality control and are involved in the pathogenesis of these diseases. The disruption of MIRO1 function can lead to mitochondrial dysfunction and energy deficits which are underlying factors in these neurodegenerative processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (ab320018), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-16-bsa-and-azide-free-capture-ab320018--sandwich-elisa-img433963.jpg "Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (ab320018)")
Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (ab320018)
Sandwich ELISA of MIRO1 Antibody Pair - BSA and Azide free ab320017 with the capture antibody ab320018 dilution at 2 ug/mL and detector antibody Anti-MIRO1 antibody [MJF-D29136-145] - BSA and Azide free (Detector) ab320019 dilution at 0.5 ug/mL.
![Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (ab320018), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-16-bsa-and-azide-free-capture-ab320018--sandwich-elisa-img433964.jpg "Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (ab320018)")
Sandwich ELISA - Anti-MIRO1 antibody [MJF-D29136-16] - BSA and Azide free (Capture) (ab320018)
Interpolated concentrations of native MIRO1 in human control wild type HeLa (human cervical adenocarcinoma epithelial cell) and RHOT1 (MIRO1) knockout HeLa cell based on 250 ug/mL extract loads. The concentrations of MIRO1 were measured in duplicate and interpolated from the MIRO1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIRO1 concentration was determined to be 3087.8 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line ab255928) and undetectable in RHOT1 (MIRO1) knockout HeLa extract (Human RHOT1 (MIRO1) knockout HeLa cell line Human RHOT1 (MIRO1) knockout HeLa cell line ab265792).
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