Knockout Tested Rabbit Recombinant Monoclonal MIRO1 antibody. Suitable for WB, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Images
![Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--indirect-elisa-img437832.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154)")
![Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--western-blot-img437829.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154)")
![Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--western-blot-img437830.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154)")
![Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--western-blot-img437831.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154)")
![Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--indirect-elisa-img439476.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (AB319154)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | I-ELISA | Flow Cyt (Intra) | ICC/IF | IHC-P | IP | IHC-Fr | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 31.25 ng/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Mitochondrial GTPase involved in mitochondrial trafficking (PubMed:12482879, PubMed:16630562, PubMed:22396657). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657). Promotes mitochondrial fission during high calcium conditions (PubMed:27716788).
Additional Targets
Rhot1
Alternative names
ARHT1, RHOT1, Mitochondrial Rho GTPase 1, MIRO-1, hMiro-1, Rac-GTP-binding protein-like protein, Ras homolog gene family member T1
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal MIRO1 antibody. Suitable for WB, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- MJF-D29136-4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MIRO1 also known as RhoT1 is a mitochondrial Rho GTPase with a molecular mass of approximately 69 kDa. It functions as a pivotal component in mitochondrial transport and dynamics. This protein localizes to the outer mitochondrial membrane and is highly expressed in various tissues particularly in the brain and muscles. MIRO1 facilitates the anchoring of mitochondria to microtubule motors enabling their movement within the cell which is essential for maintaining proper energy distribution.
- Biological function summary
MIRO1 plays a significant role in regulating mitochondrial trafficking along the cytoskeleton. It forms part of a protein complex with TRAK1/2 kinesin and dynein motor proteins. MIRO1's presence in this complex allows it to modulate mitochondrial distribution and dynamics which are essential for neural development and function. It also influences mitochondrial shape and interaction with the endoplasmic reticulum impacting intracellular calcium homeostasis.
- Pathways
MIRO1 is an integral component of pathways involving mitochondrial transport and cellular energy homeostasis. It actively participates in the regulation of calcium signaling pathways alongside related proteins like TRAK1. Additionally MIRO1's involvement in the maintenance of mitochondrial dynamics links it to mitophagy pathways. These pathways regulate the removal of damaged mitochondria a vital process for cellular health and longevity.
- Associated diseases and disorders
MIRO1 has connections to neurodegenerative conditions such as Parkinson's disease and Alzheimer's disease. These conditions correlate with impaired mitochondrial trafficking and dynamics. MIRO1 interacts with proteins such as PINK1 and Parkin which contribute to mitochondrial quality control and are involved in the pathogenesis of these diseases. The disruption of MIRO1 function can lead to mitochondrial dysfunction and energy deficits which are underlying factors in these neurodegenerative processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--indirect-elisa-img437832.jpg "Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)")
Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)
Indirect ELISA analysis of ab319154 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human RHOT1,Human RHOT2.
Antigen concentration: 1000 ng/ml
This antibody does not cross-react with human RHOT2.
![Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--western-blot-img437829.jpg "Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)")
Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
p>In Western blot, ab319154 was shown to bind specifically to RHOT1. Target of interest was observed at 80 kDa band in wild-type Hela cell lysates (lane 1) with no signal observed at this size in RHOT1 knockout cell line (lane 2) (lane 2, knockout cell line Human RHOT1 (MIRO1) knockout HeLa cell line ab265792).In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Western blot - Human RHOT1 (MIRO1) knockout HeLa cell line (Human RHOT1 (MIRO1) knockout HeLa cell line ab265792) at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Performed under reducing conditions.
Observed band size: 80 kDa, 36 kDa
Exposure time: 48s
![Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--western-blot-img437830.jpg "Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)")
Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154) at 1/1000 dilution
Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole cell lysate at 20 µg
Lane 3: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 6: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg
Lane 7: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 20 µg
Lane 8: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg
Lane 9: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 80 kDa
Exposure time: 180s
![Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--western-blot-img437831.jpg "Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)")
Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the higher MW band at approximately 150 kDa (in lane 2) is unknown.
All lanes: Western blot - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154) at 1/1000 dilution
Lane 1: Human lung tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
Lane 3: Human cerebellum tissue lysate at 20 µg
Lane 4: Mouse cerebellum tissue lysate at 20 µg
Lane 5: Mouse skeletal muscle tissue lysate at 20 µg
Lane 6: Mouse lung tissue lysate at 20 µg
Lane 7: Rat cerebellum tissue lysate at 20 µg
Lane 8: Rat skeletal muscle tissue lysate at 20 µg
Lane 9: Rat lung tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG
Observed band size: 80 kDa
Exposure time: 48s
![Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154), expandable thumbnail](https://content.abcam.com/products/images/miro1-antibody-mjf-d29136-4-ab319154--indirect-elisa-img439476.jpg "Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)")
Indirect ELISA - Anti-MIRO1 antibody [MJF-D29136-4] (ab319154)
Indirect ELISA analysis of ab319154 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Mouse RHOT1.
Antigen concentration: 1000 ng/ml
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