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AB224089

Anti-MIRO2 antibody

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(4 Publications )

Rabbit Polyclonal MIRO2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RHOT2 aa 350 to C-terminus.
6 Images
Immunocytochemistry/ Immunofluorescence - Anti-MIRO2 antibody (AB224089)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MIRO2 antibody (AB224089)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MIRO2 (green) using ab224089 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)

Immunohistochemical analysis of human cerebral cortex tissue section labeling MIRO2 with ab224089 at 1/50. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)

Immunohistochemical analysis of human skeletal muscle tissue section labeling MIRO2 with ab224089 at 1/50. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)

Immunohistochemical analysis of human heart tissue section labeling MIRO2 with ab224089 at 1/50. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody (AB224089)

Immunohistochemical analysis of human liver tissue section labeling MIRO2 with ab224089 at 1/50. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MIRO2 antibody (AB224089)
  • WB

Supplier Data

Western blot - Anti-MIRO2 antibody (AB224089)

All lanes:

Western blot - Anti-MIRO2 antibody (ab224089) at 1/100 dilution

All lanes:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RHOT2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IXI1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MIRO2 also known as Rhot2 or Mitochondrial Rho GTPase 2 is a mitochondrial Rho GTPase with a molecular weight of approximately 69 kDa. This protein belongs to the Rho family of GTPases and exists mainly on the mitochondrial outer membrane. It acts as a critical regulator of mitochondrial trafficking and dynamics. MIRO2 plays a pivotal role in the regulation of mitochondrial transport along the cytoskeleton by interacting with adaptor proteins. It features two EF-hand motifs and two GTPase domains that are essential for its function.
Biological function summary

MIRO2 contributes to the maintenance of mitochondrial network and quality within cells. It serves as a component of the calcium-sensing mitochondrial transport machinery and interacts with the transport protein complex including adaptor proteins like Milton and the motor protein kinesin. Through these interactions MIRO2 helps in coupling mitochondrial dynamics to cellular events by facilitating calcium-dependent docking and release of mitochondria. This function indicates the importance of MIRO2 in energy-demanding cellular processes.

Pathways

MIRO2 integrates into the mitochondrial transport and positioning pathways. It associates with the PINK1/Parkin pathway which is essential for mitochondrial quality control and turnover. The pathway leads to the balance of mitochondrial fission and fusion assisting in the removal of damaged mitochondria. Moreover MIRO2 collaborates with proteins like Mitofusins in these pathways to ensure efficient mitochondrial dynamics and bioenergetics.

MIRO2 has links to neurodegenerative diseases like Parkinson's disease. Dysfunction in the PINK1/Parkin pathway where MIRO2 participates leads to impaired mitophagy and neuronal cell death. Additionally imbalances in MIRO2 activity are implicated in the progression of certain types of cancer. In these contexts MIRO2 interacts with related proteins such as PINK1 and Mitofusins which play roles in disease mechanisms that involve mitochondrial dysfunction or aberrant cellular metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial GTPase involved in mitochondrial trafficking (PubMed : 16630562, PubMed : 22396657). Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (PubMed : 22396657).
See full target information RHOT2

Alternative Names

ARHT2, C16orf39, RHOT2, Mitochondrial Rho GTPase 2, MIRO-2, hMiro-2, Ras homolog gene family member T2
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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

Science advances 11:eadu5091 PubMed40203102

2025

ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Felix Kraus,João P L Coelho,Muskaan Vasandani,Jiuchun Zhang,Benjamin M Adams,Joao A Paulo,J Wade Harper,Sichen Shao

Cell reports 42:112883 PubMed37498742

2023

COPI-regulated mitochondria-ER contact site formation maintains axonal integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C Maddison,Bilal Malik,Leonardo Amadio,Dana M Bis-Brewer,Stephan Züchner,Owen M Peters,Gaynor A Smith

Respiratory research 24:99 PubMed37005656

2023

Morphologically intact airways in lung fibrosis have an abnormal proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy A Herrera,Lewis A Dingle,M Angeles Monetero,Rajamiyer V Venkateswaran,John F Blaikley,Felice Granato,Stella Pearson,Craig Lawless,David J Thornton

The Journal of cell biology 220: PubMed33891013

2021

VPS13D bridges the ER to mitochondria and peroxisomes via Miro.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Guillén-Samander,Marianna Leonzino,Michael G Hanna,Ni Tang,Hongying Shen,Pietro De Camilli
View all publications
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Product promise

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