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AB110919

Anti-MIST-1 antibody [6E8/A12/C11P1] - C-terminal

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(2 Publications)

Mouse Monoclonal MIST-1 antibody. C-terminal. Suitable for IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Bhlha15 aa 150 to C-terminus.

View Alternative Names

Bhlhb8, Mist1, Bhlha15, Class A basic helix-loop-helix protein 15, bHLHa15, Class B basic helix-loop-helix protein 8, bHLHb8, MIST-1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody [6E8/A12/C11P1] - C-terminal (AB110919)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody [6E8/A12/C11P1] - C-terminal (AB110919)

ab110919 staining MIST-1 in paraffin-embedded mouse pancreas tissue by Immunohistochemistry.

Note : Nuclear staining in acinar cells but not in duct or islet cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6E8/A12/C11P1

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Bhlha15 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9QYC3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mechanistic function of MIST-1 also known as BHLHA15 involves its role as a transcription factor. MIST-1 has a mass of approximately 29 kDa and is expressed in several tissues including the exocrine pancreas stomach and salivary glands. It is essential in regulating gene expression during cell differentiation. MIST-1 coordinates cellular activities by binding to DNA and influencing the transcription of target genes critical for secretory function in acinar cells.
Biological function summary

MIST-1 plays an important role in the development and maintenance of secretory acinar cells. It forms a complex with other transcription factors to control the genes important for cell structure and secretion. This transcription factor ensures that cells in these tissues maintain their specialized function essential for the production and release of digestive enzymes. MIST-1's function is important for maintaining cellular homeostasis and ensuring proper organ function.

Pathways

The transcriptional regulation role of MIST-1 integrates into the secretory vesicle pathway and the exocytosis pathway. It coordinates with proteins such as C/EBPα and C/EBPβ in acinar cell function influencing the secretion of digestive enzymes. MIST-1 aligns with other components within these pathways impacting the vesicular transport mechanisms that are important for proper enzyme release and digestive processes.

MIST-1 plays an important role in pancreatic diseases and gastric disorders. Its dysfunction can lead to impaired secretion and cellular pathology which are linked to conditions such as pancreatitis and gastric metaplasia. MIST-1's interaction with other proteins like C/EBPβ is critical in maintaining cellular integrity within the pancreas and disruptions in these interactions may contribute to disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated (PubMed : 17612490). Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.
See full target information Bhlha15
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed36671041

2023

Evidence of Increased Oxidative Stress in the Placental Tissue of Women Who Suffered an Episode of Psychosis during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Oscar Fraile-Martinez,Cielo García-Montero,Sonia Rodriguez-Martín,Rosa M Funes Moñux,Coral Bravo,Juan A De Leon-Luis,Jose V Saz,Miguel A Saez,Luis G Guijarro,Guillermo Lahera,Jorge Monserrat,Fernando Mora,Javier Quintero,Julia Bujan,Natalio García-Honduvilla,Melchor Alvarez-Mon,Miguel Angel Alvarez-Mon

Frontiers in cell and developmental biology 9:698292 PubMed34458260

2021

3D Organoid Culture From Adult Salivary Gland Tissues as an Modeling of Salivary Gland Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Donghyun Kim,Yeo-Jun Yoon,Dojin Choi,Jisun Kim,Jae-Yol Lim
View all publications
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Product promise

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