AB187978

Anti-MIST-1 antibody

Name: Anti-MIST-1 antibody (ab187978) | Abcam
Brand: Abcam Limited
SKU: AB187978
Price: 515 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(13 Publications)

Rabbit Polyclonal MIST-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BHLHA15 aa 100 to C-terminus.

View Alternative Names

BHLHB8, MIST1, BHLHA15, Class A basic helix-loop-helix protein 15, bHLHa15, Class B basic helix-loop-helix protein 8, bHLHb8, MIST-1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-MIST-1 antibody (AB187978)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MIST-1 antibody (AB187978)

Immuncytochemical analysis of HepG2 (Human liver hepatocellular carcinoma cell line) whole cells labeling MIST-1 in the nucleoplasm and the Golgi apparatus with ab187978 at 2 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling MIST-1 in the nucleus of exocrine glandular cells with ab187978 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MIST-1 in the striated muscle fiber (no positivity) with ab187978 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MIST-1 in the nucleus of glandular cells with ab187978 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MIST-1 in the nucleus of non-germinal center cells with ab187978 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BHLHA15 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7RTS1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mechanistic function of MIST-1 also known as BHLHA15 involves its role as a transcription factor. MIST-1 has a mass of approximately 29 kDa and is expressed in several tissues including the exocrine pancreas stomach and salivary glands. It is essential in regulating gene expression during cell differentiation. MIST-1 coordinates cellular activities by binding to DNA and influencing the transcription of target genes critical for secretory function in acinar cells.

Biological function summary

MIST-1 plays an important role in the development and maintenance of secretory acinar cells. It forms a complex with other transcription factors to control the genes important for cell structure and secretion. This transcription factor ensures that cells in these tissues maintain their specialized function essential for the production and release of digestive enzymes. MIST-1's function is important for maintaining cellular homeostasis and ensuring proper organ function.

Pathways

The transcriptional regulation role of MIST-1 integrates into the secretory vesicle pathway and the exocytosis pathway. It coordinates with proteins such as C/EBPα and C/EBPβ in acinar cell function influencing the secretion of digestive enzymes. MIST-1 aligns with other components within these pathways impacting the vesicular transport mechanisms that are important for proper enzyme release and digestive processes.

MIST-1 plays an important role in pancreatic diseases and gastric disorders. Its dysfunction can lead to impaired secretion and cellular pathology which are linked to conditions such as pancreatitis and gastric metaplasia. MIST-1's interaction with other proteins like C/EBPβ is critical in maintaining cellular integrity within the pancreas and disruptions in these interactions may contribute to disease progression and severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).

See full target information BHLHA15

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 52:492-510 PubMed40599344

2025

Extracellular vesicles derived from salivary gland stem cells cultured on microwell scaffolds loaded with WNT3A promote the recovery of salivary gland function damaged by radiation via the YWHAZ-PI3K-AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jae-Min Cho,Sujeong Ahn,Yeo-Jun Yoon,Sunyoung Park,Hyeon Song Lee,Seungyeon Hwang,Ye Jin Jeong,Yongpyo Hong,Sunyoung Seo,Dohyun Kim,Hyo-Il Jung,Won-Gun Koh,Jae-Yol Lim

Stem cell research & therapy 16:187 PubMed40251601

2025

Chemical reprogramming culture for the expansion of salivary gland epithelial basal progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Jin Jeong,Yongpyo Hong,Yeo-Jun Yoon,Nam Suk Sim,Seung-Min Hong,Jae-Yol Lim

The Journal of physiology 601:4539-4556 PubMed37724716

2023

Structural and functional analysis of salivary intercalated duct cells reveals a secretory phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda M Wahl,Takahiro Takano,Shan Su,Blake M Warner,Paola Perez,James Sneyd,David I Yule

Nature communications 14:3422 PubMed37296120

2023

Inflationary theory of branching morphogenesis in the mouse salivary gland.

Applications

Unspecified application

Species

Unspecified reactive species

Ignacio Bordeu,Lemonia Chatzeli,Benjamin D Simons

Developmental cell 58:94-109.e6 PubMed36693323

2023

A cellular hierarchy of Notch and Kras signaling controls cell fate specification in the developing mouse salivary gland.

Applications

Unspecified application

Species

Unspecified reactive species

Lemonia Chatzeli,Ignacio Bordeu,Seungmin Han,Sara Bisetto,Zahra Waheed,Bon-Kyoung Koo,Maria P Alcolea,Benjamin D Simons

Disease models & mechanisms 15: PubMed36263624

2022

Short-term and bystander effects of radiation on murine submandibular glands.

Applications

Unspecified application

Species

Unspecified reactive species

Hitoshi Uchida,Matthew H Ingalls,Eri O Maruyama,Carl J Johnston,Eric Hernady,Roberta C Faustoferri,Catherine E Ovitt

Nature communications 13:3291 PubMed35672412

2022

Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands.

Applications

Unspecified application

Species

Unspecified reactive species

Yeo-Jun Yoon,Donghyun Kim,Kwon Yong Tak,Seungyeon Hwang,Jisun Kim,Nam Suk Sim,Jae-Min Cho,Dojin Choi,Yongmi Ji,Junho K Hur,Hyunki Kim,Jong-Eun Park,Jae-Yol Lim

Cell death discovery 7:16 PubMed33462217

2021

Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands.

Applications

Unspecified application

Species

Unspecified reactive species

Abeer K Shaalan,Tathyane H N Teshima,Abigail S Tucker,Gordon B Proctor

Life science alliance 3: PubMed32345659

2020

Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn's disease model mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Shimizu,Kiminori Nakamura,Aki Yoshii,Yuki Yokoi,Mani Kikuchi,Ryuga Shinozaki,Shunta Nakamura,Shuya Ohira,Rina Sugimoto,Tokiyoshi Ayabe

FEBS letters 594:376-382 PubMed31538335

2019

Intrinsic mitotic activity supports the human salivary gland acinar cell population.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew H Ingalls,Andrew J Hollomon,Shawn D Newlands,Andrew N McDavid,Catherine E Ovitt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MIST-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MIST-1 antibody (AB187978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (AB187978)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise