Rabbit Polyclonal MIST-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BHLHA15 aa 100 to C-terminus.
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- The Journal of physiology 601:4539-45562023Structural and functional analysis of salivary intercalated duct cells reveals a secretory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmanda M Wahl,Takahiro Takano,Shan Su,Blake M Warner,Paola Perez,James Sneyd,David I YulePubMed 37724716
- Nature communications 14:34222023Inflationary theory of branching morphogenesis in the mouse salivary gland.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIgnacio Bordeu,Lemonia Chatzeli,Benjamin D SimonsPubMed 37296120
- Developmental cell 58:94-109.e62023A cellular hierarchy of Notch and Kras signaling controls cell fate specification in the developing mouse salivary gland.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLemonia Chatzeli,Ignacio Bordeu,Seungmin Han,Sara Bisetto,Zahra Waheed,Bon-Kyoung Koo,Maria P Alcolea,Benjamin D SimonsPubMed 36693323
- Disease models & mechanisms 15:2022Short-term and bystander effects of radiation on murine submandibular glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHitoshi Uchida,Matthew H Ingalls,Eri O Maruyama,Carl J Johnston,Eric Hernady,Roberta C Faustoferri,Catherine E OvittPubMed 36263624
- Nature communications 13:32912022Salivary gland organoid culture maintains distinct glandular properties of murine and human major salivary glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeo-Jun Yoon,Donghyun Kim,Kwon Yong Tak,Seungyeon Hwang,Jisun Kim,Nam Suk Sim,Jae-Min Cho,Dojin Choi,Yongmi Ji,Junho K Hur,Hyunki Kim,Jong-Eun Park,Jae-Yol LimPubMed 35672412
- Cell death discovery 7:162021Inhibition of Aurora Kinase B activity disrupts development and differentiation of salivary glands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbeer K Shaalan et. al.PubMed 33462217
- Life science alliance 3:2020Paneth cell α-defensin misfolding correlates with dysbiosis and ileitis in Crohn's disease model mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Shimizu et. al.PubMed 32345659
- FEBS letters 594:376-3822019Intrinsic mitotic activity supports the human salivary gland acinar cell population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew H Ingalls,Andrew J Hollomon,Shawn D Newlands,Andrew N McDavid,Catherine E OvittPubMed 31538335
- Scientific reports 9:113392019Salivary glands require Aurora Kinase B for regeneration after transient innate immune-mediated injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbeer Shaalan et. al.PubMed 31383943
- Cell reports 24:1464-1470.e32018Limited Regeneration of Adult Salivary Glands after Severe Injury Involves Cellular Plasticity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPei-Lun Weng,Marit H Aure,Takamitsu Maruyama,Catherine E OvittPubMed 30089258
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BHLHA15 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7RTS1
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
Associated Products
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Target data
Function
Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).
Alternative names
BHLHB8, MIST1, BHLHA15, Class A basic helix-loop-helix protein 15, bHLHa15, Class B basic helix-loop-helix protein 8, bHLHb8, MIST-1
Recommended products
Rabbit Polyclonal MIST-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BHLHA15 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BHLHA15 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q7RTS1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mechanistic function of MIST-1 also known as BHLHA15 involves its role as a transcription factor. MIST-1 has a mass of approximately 29 kDa and is expressed in several tissues including the exocrine pancreas stomach and salivary glands. It is essential in regulating gene expression during cell differentiation. MIST-1 coordinates cellular activities by binding to DNA and influencing the transcription of target genes critical for secretory function in acinar cells.
- Biological function summary
MIST-1 plays an important role in the development and maintenance of secretory acinar cells. It forms a complex with other transcription factors to control the genes important for cell structure and secretion. This transcription factor ensures that cells in these tissues maintain their specialized function essential for the production and release of digestive enzymes. MIST-1's function is important for maintaining cellular homeostasis and ensuring proper organ function.
- Pathways
The transcriptional regulation role of MIST-1 integrates into the secretory vesicle pathway and the exocytosis pathway. It coordinates with proteins such as C/EBPα and C/EBPβ in acinar cell function influencing the secretion of digestive enzymes. MIST-1 aligns with other components within these pathways impacting the vesicular transport mechanisms that are important for proper enzyme release and digestive processes.
- Associated diseases and disorders
MIST-1 plays an important role in pancreatic diseases and gastric disorders. Its dysfunction can lead to impaired secretion and cellular pathology which are linked to conditions such as pancreatitis and gastric metaplasia. MIST-1's interaction with other proteins like C/EBPβ is critical in maintaining cellular integrity within the pancreas and disruptions in these interactions may contribute to disease progression and severity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling MIST-1 in the nucleus of exocrine glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MIST-1 antibody (ab187978)
Immuncytochemical analysis of HepG2 (Human liver hepatocellular carcinoma cell line) whole cells labeling MIST-1 in the nucleoplasm and the Golgi apparatus with ab187978 at 2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MIST-1 in the nucleus of non-germinal center cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling MIST-1 in the nucleus of glandular cells with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIST-1 antibody (ab187978)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling MIST-1 in the striated muscle fiber (no positivity) with ab187978 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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