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AB122982

Anti-MiTF antibody

1

(1 Review)

|

(20 Publications)

Rabbit Polyclonal MITF antibody. Suitable for ICC/IF, IHC-Fr and reacts with Mouse, Chicken samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MITF.

View Alternative Names

BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody (AB122982)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody (AB122982)

Immunofluorescence analysis of mouse primary melanocytes labelling MiTF with ab122982 at a dilution of 1/300 (right). Nuclei couterstained with DAPI (blue) (left).

Immunohistochemistry (Frozen sections) - Anti-MiTF antibody (AB122982)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MiTF antibody (AB122982)

Immunohistochemical analysis of RPE (Retinal Pigment Epithelium) in chicken embryo (stage 14) labelling MiTF with ab122982 at dilution of 1/300. Embryo was fixed with paraformaldehyde and embedded in OCT compound and sectioned with a cryostat at 8
μm. Alexa Fluor® 488 conjugated anti-rabbit IgG was used at the secondary antibody.

At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Chicken

Applications

ICC/IF, IHC-Fr

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MITF.

O75030

Specificity

ab122982 works well with the eye.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/300", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/300 - 1/1000", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.05% Sodium azide Constituents: 99% Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed : 36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed : 36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758).
See full target information MITF
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:87 PubMed40556005

2025

FRRS1L variants and ferriheme overload drive hyperpigmentation and systemic Iron overload in lanping black bone sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Deping Han,Yuanyuan Zhang,Weidong Deng,Xue Yang,Jianfei Chen,Guoying Hua,Hesham Y A Darwish,Huaming Mao,Xiao Gou,Jiankui Wang,Kai Cui,Yuhao Ma,Yurong Tai,Xianggui Dong,Yanzhu Yao,Zu Yang,Suying Cao,Zhengquan Yu,Wansheng Liu,Xuemei Deng

Animals : an open access journal from MDPI 13: PubMed37627399

2023

Effects of SLC45A2 and GPNMB on Melanin Deposition Based on Transcriptome Sequencing in Chicken Feather Follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiting Li,Yanxing Wang,Yihan Liu,Donghua Li,Yadong Tian,Xiaojun Liu,Xiangtao Kang,Zhuanjian Li

Cell reports 42:112335 PubMed37027294

2023

Neurons require glucose uptake and glycolysis in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Li,Caroline Guglielmetti,Yoshitaka J Sei,Misha Zilberter,Lydia M Le Page,Lauren Shields,Joyce Yang,Kevin Nguyen,Brice Tiret,Xiao Gao,Neal Bennett,Iris Lo,Talya L Dayton,Martin Kampmann,Yadong Huang,Jeffrey C Rathmell,Matthew Vander Heiden,Myriam M Chaumeil,Ken Nakamura

Nature protocols 17:1266-1305 PubMed35322210

2022

Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoon Lee,Wouter H van der Valk,Sara A Serdy,CiCi Deakin,Jin Kim,Anh Phuong Le,Karl R Koehler

Toxicology reports 9:670-678 PubMed35433274

2022

The effect of docetaxel on retinal pigment epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jialin Li,Xiao Liu,Qiaohui Yang,Jian Huang,Wang Zhou,Zhirong Tan,Zhuo Li,Di Zhou

Cancers 14: PubMed35159049

2022

Uveal Melanoma Cell Line Proliferation Is Inhibited by Ricolinostat, a Histone Deacetylase Inhibitor.

Applications

Unspecified application

Species

Unspecified reactive species

Husvinee Sundaramurthi,Sandra García-Mulero,Valentina Tonelotto,Kayleigh Slater,Simone Marcone,Josep M Piulats,Ronald William Watson,Desmond J Tobin,Lasse D Jensen,Breandán N Kennedy

Experimental & molecular medicine 53:631-642 PubMed33828232

2021

Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jee Myung Yang,Sunho Chung,KyungA Yun,Bora Kim,Seongjun So,Seoon Kang,Eunju Kang,Joo Yong Lee

International journal of molecular sciences 22: PubMed33802460

2021

GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Martijn J C van der Lienden,Jan Aten,André R A Marques,Ingeborg S E Waas,Per W B Larsen,Nike Claessen,Nicole N van der Wel,Roelof Ottenhoff,Marco van Eijk,Johannes M F G Aerts

OncoTargets and therapy 13:12457-12469 PubMed33299329

2020

α-MSH-PE38KDEL Kills Melanoma Cells via Modulating Erk1/2/MITF/TYR Signaling in an MC1R-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Liu,Hong Li,Xianling Cong,Da Huo,Lele Cong,Guangzhi Wu

Genes & development 34:1713-1734 PubMed33184221

2020

BMP signaling: at the gate between activated melanocyte stem cells and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole R Infarinato,Katherine S Stewart,Yihao Yang,Nicholas C Gomez,H Amalia Pasolli,Lynette Hidalgo,Lisa Polak,Thomas S Carroll,Elaine Fuchs
View all publications
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Product promise

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