Rabbit Polyclonal MITF antibody. Suitable for ICC/IF, IHC-Fr and reacts with Mouse, Chicken samples. Cited in 17 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MITF.
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- Cell reports 42:1123352023Neurons require glucose uptake and glycolysis in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuihui Li,Caroline Guglielmetti,Yoshitaka J Sei,Misha Zilberter,Lydia M Le Page,Lauren Shields,Joyce Yang,Kevin Nguyen,Brice Tiret,Xiao Gao,Neal Bennett,Iris Lo,Talya L Dayton,Martin Kampmann,Yadong Huang,Jeffrey C Rathmell,Matthew Vander Heiden,Myriam M Chaumeil,Ken NakamuraPubMed 37027294
- Nature protocols 17:1266-13052022Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiyoon Lee,Wouter H van der Valk,Sara A Serdy,CiCi Deakin,Jin Kim,Anh Phuong Le,Karl R KoehlerPubMed 35322210
- Toxicology reports 9:670-6782022The effect of docetaxel on retinal pigment epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJialin Li,Xiao Liu,Qiaohui Yang,Jian Huang,Wang Zhou,Zhirong Tan,Zhuo Li,Di ZhouPubMed 35433274
- Cancers 14:2022Uveal Melanoma Cell Line Proliferation Is Inhibited by Ricolinostat, a Histone Deacetylase Inhibitor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHusvinee Sundaramurthi,Sandra García-Mulero,Valentina Tonelotto,Kayleigh Slater,Simone Marcone,Josep M Piulats,Ronald William Watson,Desmond J Tobin,Lasse D Jensen,Breandán N KennedyPubMed 35159049
- Experimental & molecular medicine 53:631-6422021Long-term effects of human induced pluripotent stem cell-derived retinal cell transplantation in Pde6b knockout rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJee Myung Yang,Sunho Chung,KyungA Yun,Bora Kim,Seongjun So,Seoon Kang,Eunju Kang,Joo Yong LeePubMed 33828232
- OncoTargets and therapy 13:12457-124692020α-MSH-PE38KDEL Kills Melanoma Cells via Modulating Erk1/2/MITF/TYR Signaling in an MC1R-Dependent Manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXilin Liu et. al.PubMed 33299329
- Genes & development 34:1713-17342020BMP signaling: at the gate between activated melanocyte stem cells and differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicole R Infarinato et. al.PubMed 33184221
- Frontiers in bioengineering and biotechnology 8:5402020Human Melanocyte-Derived Spheroids: A Precise Test System for Drug Screening and a Multicellular Unit for Tissue Engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrina M Zurina et. al.PubMed 32582665
- Stem cell research & therapy 10:2492019Differentiation of adipose-derived stem cells to functional CD105 CD73 melanocyte precursors guided by defined culture condition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela Zavala et. al.PubMed 31399041
- Frontiers in cellular neuroscience 13:3612019Modeling Retinitis Pigmentosa: Retinal Organoids Generated From the iPSCs of a Patient With the USH2A Mutation Show Early Developmental Abnormalities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYonglong Guo et. al.PubMed 31481876
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.05% Sodium azide
Constituents: 99% Whole serum- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MITF. Database link O75030
Reactivity data
Select an application| ICC/IF | IHC-Fr | |
|---|---|---|
| Mouse | Tested | Expected |
| Chicken | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/300 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info 1/300.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed:36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed:36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed:10587587, PubMed:22647378, PubMed:27889061, PubMed:9647758).
Alternative names
BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
Recommended products
Rabbit Polyclonal MITF antibody. Suitable for ICC/IF, IHC-Fr and reacts with Mouse, Chicken samples. Cited in 17 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MITF.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.05% Sodium azide
Constituents: 99% Whole serum- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MITF. Database link O75030
- Purity
- Whole antiserum
- Specificity
ab122982 works well with the eye.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Frozen sections) - Anti-MiTF antibody (ab122982)
Immunohistochemical analysis of RPE (Retinal Pigment Epithelium) in chicken embryo (stage 14) labelling MiTF with ab122982 at dilution of 1/300. Embryo was fixed with paraformaldehyde and embedded in OCT compound and sectioned with a cryostat at 8
μm. Alexa Fluor® 488 conjugated anti-rabbit IgG was used at the secondary antibody.At stage 14, Mitif protein is detected throughout the RPE (Retinal Pigment Epithelium).
Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody (ab122982)
Immunofluorescence analysis of mouse primary melanocytes labelling MiTF with ab122982 at a dilution of 1/300 (right). Nuclei couterstained with DAPI (blue) (left).
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