Rabbit Polyclonal MITF antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 44 publications.
Images
Publications
- International journal of molecular sciences 24:2023Generating Functional and Highly Proliferative Melanocytes Derived from Human Pluripotent Stem Cells: A Promising Tool for Biotherapeutic Approaches to Treat Skin Pigmentation Disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManoubia Saidani,Annabelle Darle,Margot Jarrige,Hélène Polveche,Lina El Kassar,Séverine Julié,Sandrine Bessou-Touya,Nathalie Holic,Gilles Lemaitre,Cécile Martinat,Christine Baldeschi,Jennifer AllouchePubMed 37047372
- Frontiers in pharmacology 14:10810302023Cryptochrome 1 activation inhibits melanogenesis and melanosome transport through negative regulation of cAMP/PKA/CREB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongyin Gao,Ximei Zhang,Kun Zou,Duo Meng,Jinpeng LvPubMed 36814484
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC (PFA fixed) | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
| Rat | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
IHC (PFA fixed)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Alternative names
Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, BHLHE32, MITF
Recommended products
Rabbit Polyclonal MITF antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 44 publications.
Alternative names
Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, BHLHE32, MITF
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-MiTF antibody (ab20663)
All lanes: Western blot - Anti-MiTF antibody (ab20663) at 1 µg/mL
Lanes 1 and 4: HeLa whole cell lysate at 20 µg
Lanes 2 and 5: HeLa nuclear lysate at 20 µg
Lanes 3 and 6: A431 whole cell lysate at 20 µg
SecondaryAll lanes: Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 25 kDa, 37 kDa, 52 kDa
Western blot - Anti-MiTF antibody (ab20663)
All lanes: Western blot - Anti-MiTF antibody (ab20663) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Brain (Mouse) Tissue Lysate at 10 µg
Lane 4: Testis (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: Spinal Cord (Mouse) Tissue Lysate at 10 µg
Lane 6: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 7: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 8: Brain (Rat) Tissue Lysate - normal tissue at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 52 kDa
Downloads
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