Anti-MiTF antibody

• WB

Project

Western blot - Anti-MiTF antibody (AB20663)

All lanes:

Western blot - Anti-MiTF antibody (ab20663) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/en-us/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 4:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 5:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 6:

Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 7:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Lane 8:

Brain (Rat) Tissue Lysate - normal tissue at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 52 kDa

false

• WB

Project

Western blot - Anti-MiTF antibody (AB20663)

All lanes:

Western blot - Anti-MiTF antibody (ab20663) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 20 µg

Lane 2:

HeLa nuclear lysate at 20 µg

Lane 3:

A431 whole cell lysate at 20 µg

Lane 4:

HeLa whole cell lysate at 20 µg with Human MiTF peptide (<a href='/en-us/products/proteins-peptides/human-mitf-peptide-ab21454'>ab21454</a>)

Lane 5:

HeLa nuclear lysate at 20 µg with Human MiTF peptide (<a href='/en-us/products/proteins-peptides/human-mitf-peptide-ab21454'>ab21454</a>)

Lane 6:

A431 whole cell lysate at 20 µg with Human MiTF peptide (<a href='/en-us/products/proteins-peptides/human-mitf-peptide-ab21454'>ab21454</a>)

Secondary

All lanes:

Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

Predicted band size: 58 kDa

Observed band size: 25 kDa,37 kDa,52 kDa

false