Anti-MITF Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MITF Antibody (AB327178)

Immunohistochemistry analysis in human skin and pancreas tissues using ab327178 at 1 : 500–1 : 1000 dilution. Corresponding MITF RNA-seq data are presented for the same tissues.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MITF Antibody (AB327178)

Immunohistochemical staining of human endometrium using ab327178 at 1 : 500–1 : 1000 dilution, showing moderate nuclear positivity in cells in endometrial stroma.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MITF Antibody (AB327178)

Immunohistochemical staining of human skin using ab327178 at 1 : 500–1 : 1000 dilution, showing moderate to strong nuclear positivity in melanocytes.

• ChIP

Supplier Data

ChIP - Anti-MITF Antibody (AB327178)

ChIP-Exo-Seq composite graph for ab327178 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-MITF Antibody (AB327178)

Western blot analysis in human cell lines SK-MEL-30 and MCF-7 using ab327178 at 0.04–0.4 µg/ml. Corresponding MITF RNA-seq data are presented for the same cell lines. Loading control : Anti-PFN1.

All lanes:

Western blot - Anti-MITF Antibody (ab327178)

Lane 2:

Human cell lines SK-MEL-30

Lane 3:

Human cell lines MCF-7

Predicted band size: 58.8 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MITF Antibody (AB327178)

Immunohistochemical staining of human liver using ab327178 at 1 : 500–1 : 1000 dilution, showing no positivity in hepatocytes as expected.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MITF Antibody (AB327178)

Immunohistochemical staining of human pancreas using ab327178 at 1 : 500–1 : 1000 dilution, showing no positivity in exocrine glandular cells as expected.