AB12039

Anti-MiTF antibody [C5]

Name: Anti-MiTF antibody [C5] (ab12039) | Abcam
Brand: Abcam Limited
SKU: AB12039
Price: 520 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(128 Publications)

Anti-MiTF antibody [C5] (ab12039) is a mouse monoclonal antibody detecting MiTF in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Over 110 publications
- Trusted since 2004

View Alternative Names

BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32

3 Images

Project8027****

Western blot - Anti-MiTF antibody [C5] (AB12039)

All lanes:

Western blot - Anti-MiTF antibody [C5] (ab12039) at 1 µg/mL

All lanes:

Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 58 kDa

Observed band size: 40 kDa,55 kDa,70 kDa

true

Exposure time: 20min

Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (AB12039)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (AB12039)

Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (AB12039)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (AB12039)

ab12039 staining MiTF in Malme-3M cells. The cells were permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab12039 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-MiTF antibody [C5] (ab12039) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of MiTF?
Anti-MiTF [C5] (ab12039) specifically detects a band for MiTF (UniProt: O75030) at a molecular weight of 59kDa.

Trusted by the scientific community
Anti-MiTF [C5] (ab12039) was first used in a scientific publication in 2004 and has been cited over 110 times in peer-reviewed journals.

Reviewed by scientists
Anti-MiTF [C5] (ab12039) has over 5 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [C5] also available for your convenience: ab12039, Carrier free - ab264110

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed : 36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed : 36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758).

See full target information MITF

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Publications (128)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology : PubMed41028831

2025

Derivation of embryonic stem cells across avian species.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Zheng Guo,Xinyi Tong,Xizi Wang,Xugeng Liu,Hiroki Nagai,Ping Wu,Jiayi Lu,David Huss,Martin Tran,Carol Readhead,Christina Wu,Lin Cao,Yixin Huang,Zhaohan Zeng,Fan Feng,Nima Adhami,Sirjan Mor,Rusty Lansford,Cheng-Ming Chuong,Guojun Sheng,Carlos Lois,Qi-Long Ying

PLoS genetics 21:e1011693 PubMed40354322

2025

Molecular insights into region-specific sexual dichromatism: Comparative transcriptome analysis of red cheek pigmentation in zebra finches.

Applications

Unspecified application

Species

Unspecified reactive species

Gee-Way Lin,Chih-Kuan Chen,Ting-Xin Jiang,Ya-Chen Liang,Pin-Chi Tang,Ping Wu,Randall B Widelitz,Chih-Feng Chen,Cheng-Ming Chuong

The Journal of clinical investigation 135: PubMed40311102

2025

Tebentafusp elicits on-target cutaneous immune responses driven by cytotoxic T cells in uveal melanoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ramon Staeger,Aizhan Tastanova,Adhideb Ghosh,Nicola Winkelbeiner,Prachi Shukla,Isabel Kolm,Patrick Turko,Adel Benlahrech,Jane Harper,Anna Broomfield,Antonio Camera,Marianna Ambrosio,Veronika Haunerdinger,Phil F Cheng,Egle Ramelyte,James Pham,Stefanie Kreutmair,Burkhard Becher,Mitchell P Levesque,Reinhard Dummer,Barbara Meier-Schiesser

Regenerative therapy 26:749-759 PubMed39290629

2024

Efficient and cost-effective differentiation of induced neural crest cells from induced pluripotent stem cells using laminin 211.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuma Takahashi,Shizuka Aritomi,Fumie Honkawa,Sayaka Asari,Ken Hirose,Atsushi Konishi

EMBO reports 25:4252-4280 PubMed39169200

2024

Novel mechanisms of MITF regulation identified in a mouse suppressor screen.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Nhung Vu,Matti Már Valdimarsson,Sara Sigurbjörnsdóttir,Kristín Bergsteinsdóttir,Julien Debbache,Keren Bismuth,Deborah A Swing,Jón H Hallsson,Lionel Larue,Heinz Arnheiter,Neal G Copeland,Nancy A Jenkins,Petur O Heidarsson,Eiríkur Steingrímsson

Cell reports 43:114484 PubMed38990725

2024

Feedforward cysteine regulation maintains melanoma differentiation state and limits metastatic spread.

Applications

Unspecified application

Species

Unspecified reactive species

Deyang Yu,Jiaxin Liang,Hans R Widlund,Pere Puigserver

International journal of biological sciences 20:1688-1704 PubMed38481807

2024

Interruption of p38-MSK1-CREB-MITF-M pathway to prevent hyperpigmentation in the skin.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Hee Kim,Jiyeon Lee,Jihye Jung,Ga Hyun Kim,Cheong-Yong Yun,Sang-Hun Jung,Bang Yeon Hwang,Jin Tae Hong,Sang-Bae Han,Jae-Kyung Jung,Youngsoo Kim

Cell death & disease 15:208 PubMed38472212

2024

The MITF/mir-579-3p regulatory axis dictates BRAF-mutated melanoma cell fate in response to MAPK inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Domenico Liguoro,Rachele Frigerio,Arianna Ortolano,Andrea Sacconi,Mario Acunzo,Giulia Romano,Giovanni Nigita,Barbara Bellei,Gabriele Madonna,Mariaelena Capone,Paolo Antonio Ascierto,Rita Mancini,Gennaro Ciliberto,Luigi Fattore

International journal of biological sciences 20:312-330 PubMed38164184

2024

Targeting phosphorylation circuits on CREB and CRTCs as the strategy to prevent acquired skin hyperpigmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Hee Kim,Changseon Na,Cheng-Yong Yun,Jun Gu Kim,Seung Tae Baek,Hyun Jin An,Jae Duk Lee,Seung Wha Lee,Jae-Kyung Jung,Bang Yeon Hwang,Sang-Bae Han,Youngoo Kim

Frontiers in cell and developmental biology 11:1252547 PubMed37691820

2023

Integration of human stem cell-derived systems and mouse preclinical models identifies complex pathophysiologic mechanisms in retinal dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa K Jones,Luz D Orozco,Han Qin,Tom Truong,Patrick Caplazi,Justin Elstrott,Zora Modrusan,Shawnta Y Chaney,Marion Jeanne

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MiTF antibody [C5]

Western blot - Anti-MiTF antibody [C5] (AB12039)

Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (AB12039)

Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (AB12039)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (128)

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