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Mouse Monoclonal MITF antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Human samples. Cited in 113 publications.


Images

Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (AB12039), expandable thumbnail
  • Western blot - Anti-MiTF antibody [C5] (AB12039), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (AB12039), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow Cyt (Intra)WB
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Chicken
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2 µg for 106 Cells

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse, Rat, Chicken

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken

Dilution info

-

Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

1 product for Alternative Version

Target data

Function

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Alternative names

Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32, BHLHE32, MITF

Recommended products

Mouse Monoclonal MITF antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB and reacts with Human samples. Cited in 113 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

C5

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (ab12039), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MiTF antibody [C5] (ab12039)

    Overlay histogram showing Malme 3m cells stained with ab12039 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12039, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-MiTF antibody [C5] (ab12039), expandable thumbnail

    Western blot - Anti-MiTF antibody [C5] (ab12039)

    All lanes: Western blot - Anti-MiTF antibody [C5] (ab12039) at 1 µg/mL

    All lanes: Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa

    Observed band size: 40 kDa, 55 kDa, 70 kDa

    Exposure time: 20min

  • Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (ab12039), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [C5] (ab12039)

    ab12039 staining MiTF in Malme-3M cells. The cells were permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab12039 at 5µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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