Anti-MiTF antibody [D5] - BSA and Azide free

• ICC

Lab

Immunocytochemistry - Anti-MiTF antibody [D5] - BSA and Azide free (AB182842)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3201).

ab3201 staining MiTF in Malme-3M cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab3201 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MiTF antibody [D5] - BSA and Azide free (AB182842)

Overlay histogram showing Malme-3 cells stained with ab3201 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3201, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab3201).

• WB

Supplier Data

Western blot - Anti-MiTF antibody [D5] - BSA and Azide free (AB182842)

This data was developed using ab127540, the same antibody clone in a different buffer formulation.

7.5% SDS-PAGE

All lanes:

Anti-ZNF282 antibody (<a href='/en-us/products/unavailable/znf282-antibody-ab127540'>ab127540</a>) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 58 kDa

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