Anti-MiTF antibody [EPR26363-10]

9 product images

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  Western blot - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Blocking and dilution buffer and concentration: 5% NFDM/TBST
  

  Exposure time:

  Lanes 1, 3, 4: 70 seconds

  Lane 2: 180 seconds

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  The molecular weight observed is consistent with what has been described in the literature (PMID: 17182576).

  All lanes: Western blot - Anti-MiTF antibody [EPR26363-10] (ab303530) at 1/1000 dilution

  Lane 1: SK-MEL-28 (human malignant melanoma), whole cell lysate at 20 µg

  Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 3: B16F0 (mouse melanoma epithelial cell-like), whole cell lysate at 20 µg

  Lane 4: B16F10 (mouse skin melanoma cell), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Predicted band size: 58 kDa

  Observed band size: 40-70 kDa

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  Flow Cytometry (Intracellular) - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SK-MEL-28 (human malignant melanoma) cells labelling MiTF with ab303530 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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  Flow Cytometry (Intracellular) - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized B16F0 (mouse melanoma epithelial cell-like) cells labelling MiTF with ab303530 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized B16-F10 (mouse skin melanoma cell) cells labelling MiTF with ab303530 at 1/50 (11.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing mostly nuclear staining in B16-F0 cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling MiTF with ab303530 at 1/50 (11.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing mostly nuclear staining in SK-MEL-28 cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling MiTF with ab303530 at 1/5000 (0.113 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse skin. The section was incubated with ab303530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling MiTF with ab303530 at 1/5000 (0.113 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human melanoma. The section was incubated with ab303530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Immunohistochemical analysis of paraffin-embedded Rat embryonic eye tissue labeling MiTF with ab303530 at 1/5000 (0.113 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat embryonic eye. The section was incubated with ab303530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MiTF antibody [EPR26363-10] (ab303530)

  Immunohistochemical analysis of paraffin-embedded Mouse embryonic eye tissue labeling MiTF with ab303530 at 1/5000 (0.113 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse embryonic eye. The section was incubated with ab303530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins