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AB59201

Anti-MiTF (phospho S73) antibody

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(7 Publications )

Rabbit Polyclonal MITF phospho S73 antibody. Suitable for WB and reacts with African green monkey samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MITF pS73 aa 150-200.
1 Images
Western blot - Anti-MiTF (phospho S73) antibody (AB59201)
  • WB

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Western blot - Anti-MiTF (phospho S73) antibody (AB59201)

All lanes:

Western blot - Anti-MiTF (phospho S73) antibody (ab59201) at 1/500 dilution

Lane 1:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract

Lane 2:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract with phosphopeptide

Predicted band size: 58 kDa

Observed band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

African green monkey

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MITF pS73 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

O75030

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Involved in the cellular response to amino acid availability by acting downstream of MTOR : in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation (PubMed : 36608670). Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity (PubMed : 36608670). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium (PubMed : 10587587, PubMed : 22647378, PubMed : 27889061, PubMed : 9647758).
See full target information MITF pS73

Alternative Names

BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 34:985-986 PubMed38668686

2024

Corrigendum to: Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yujin Kim,Jung Ok Lee,Jung Min Lee,Mun-Hoe Lee,Hyeong-Min Kim,Hee-Chul Chung,Do-Un Kim,Jin-Hee Lee,Beom Joon Kim

Journal of microbiology and biotechnology 34:17-28 PubMed37830229

2023

Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3β/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yujin Kim,Jung Ok Lee,Jung Min Lee,Mun-Hoe Lee,Hyeong-Min Kim,Hee-Chul Chung,Do-Un Kim,Jin-Hee Lee,Beom Joon Kim

Journal of ginseng research 47:714-725 PubMed38107393

2023

Phenolic acids in inhibit melanin production through bidirectional regulation of melanin synthase transcription via different signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzeng Liu,Xiaohao Xu,Jingyuan Zhou,Guang Sun,Zhenzhuo Li,Lu Zhai,Jing Wang,Rui Ma,Daqing Zhao,Rui Jiang,Liwei Sun

The British journal of dermatology 185:391-404 PubMed33400324

2021

Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Y Lan,W Zeng,X Dong,H Lu

OncoTargets and therapy 13:12457-12469 PubMed33299329

2020

α-MSH-PE38KDEL Kills Melanoma Cells via Modulating Erk1/2/MITF/TYR Signaling in an MC1R-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Liu,Hong Li,Xianling Cong,Da Huo,Lele Cong,Guangzhi Wu

Oncotarget 8:95530-95541 PubMed29221146

2017

Swertiajaponin inhibits skin pigmentation by dual mechanisms to suppress tyrosinase.

Applications

Unspecified application

Species

Unspecified reactive species

Bonggi Lee,Kyoung Mi Moon,Bong-Seon Lee,Ju-Hye Yang,Kwang Il Park,Won-Kyung Cho,Jin Yeul Ma

Pathobiology : journal of immunopathology, molecul 82:269-79 PubMed26516773

2015

Evaluation of Fibroblast Growth Factor Receptor 2 Expression, Heterogeneity and Clinical Significance in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nayoung Han,Min A Kim,Hye Seung Lee,Woo Ho Kim
View all publications
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