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AB214205

Anti-Mitochondrial dicarboxylate carrier antibody

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(1 Publication)

Rabbit Polyclonal Mitochondrial dicarboxylate carrier antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC25A10 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

DIC, SLC25A10, Mitochondrial dicarboxylate carrier, Solute carrier family 25 member 10

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial dicarboxylate carrier antibody (AB214205)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial dicarboxylate carrier antibody (AB214205)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Mitochondrial dicarboxylate carrier with ab214205 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC25A10 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UBX3

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Complementary Products

Cell Lines & Lysates

AB266122

Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell line

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Cell Lines & Lysates

AB259118

Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell lysate

Sanger Sequencing - Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell lysate (AB259118)

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Primary Antibodies

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Primary Antibodies

AB169528

Anti-eIF2A antibody [EPR11042]

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Immunocytochemistry/ Immunofluorescence - Anti-eIF2A antibody [EPR11042] (AB169528)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitochondrial dicarboxylate carrier also known as the dicarboxylate transporter facilitates the exchange of dicarboxylates across the mitochondrial inner membrane. This carrier protein often termed the dicarboxylate carrier or oxodicarboxylate transporter possesses a molecular mass of approximately 31 kilodaltons and is expressed predominantly in metabolically active tissues such as liver kidney and heart. Its key function lies in transporting four-carbon dicarboxylates like succinate and malate important for energy and metabolic equilibrium.
Biological function summary

The mitochondrial dicarboxylate carrier aids in mitochondrial processes by mediating the shuttle of dicarboxylates important for the tricarboxylic acid (TCA) cycle and urea cycle. It actively participates in the transfer of metabolites that contribute to the synthesis of ATP linking important metabolic routes. The dicarboxylate carrier operates as a part of the family of mitochondrial transport proteins but does not form part of a larger protein complex.

Pathways

The dicarboxylate carrier intersects several key metabolic pathways including the TCA cycle and gluconeogenesis. Through these pathways the protein collaborates with others like the adenine nucleotide translocase; it aids in maintaining energetic homeostasis within cells. The correct functioning of the dicarboxylate carrier ensures the proper flow of carbon skeletons essential for cellular respiration and energy production.

The malfunction of the mitochondrial dicarboxylate carrier links to metabolic conditions like renal tubular acidosis and certain forms of mitochondrial myopathy. The carrier's dysfunction may result in disrupted acid-base homeostasis within the kidneys due to impaired succinate transport. In these contexts proteins such as the mitochondrial pyruvate carrier may exhibit interconnected roles further influencing pathological states. Understanding the carrier's activity impacts strategies for addressing metabolic dysregulations associated with mitochondrial diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the electroneutral exchange or flux of physiologically important metabolites such as dicarboxylates (malonate, malate, succinate), inorganic sulfur-containing anions, and phosphate, across the mitochondrial inner membrane (PubMed : 29211846, PubMed : 38780415, PubMed : 38937634). Substrate exchange across the membrane occurs consecutively with one substrate being transported first, then dissociating from the substrate binding site before the second substrate binds for transport in the opposite direction (PubMed : 38937634). Does not transport glutathione (By similarity). Plays an important role in gluconeogenesis, fatty acid metabolism, urea synthesis, and sulfur metabolism, particularly in liver, by supplying the substrates for the different metabolic processes. Regulates fatty acid release from adipocytes, and contributes to systemic insulin sensitivity (By similarity).
See full target information SLC25A10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73:1859-1873 PubMed39235608

2024

Stored RBC transfusions leads to the systemic inflammatory response syndrome in anemic murine neonates.

Applications

Unspecified application

Species

Unspecified reactive species

Balamurugan Ramatchandirin,Marie Amalie Balamurugan,Suneetha Desiraju,Yerin Chung,Boguslaw S Wojczyk,Krishnan MohanKumar
View all publications
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Product promise

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