Anti-Mitochondrial Ferritin antibody [EPR1797]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (AB124889)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Mitochondrial Ferritin with ab124889 at 1/5000 dilution (0.03 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positve staining on the human testis. The section was incubated with ab124889 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Lab

Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (AB124889)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : human cerebellum. In Western blot, anti-Anti-beta Tubulin antibody (ab52901) staining at 1/20000 dilution.

All lanes:

Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 28 kDa

Observed band size: 21 kDa

false

Exposure time: 180s

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial Ferritin antibody [EPR1797] (AB124889)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Mitochondrial Ferritin with ab124889 at 1/5000 dilution (0.03 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. The section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Negative control : no staining on the human cerebrum. The section was incubated with ab124889 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Unknown

Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (AB124889)

All lanes:

Western blot - Anti-Mitochondrial Ferritin antibody [EPR1797] (ab124889) at 1/1000 dilution

All lanes:

Human Testis lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 28 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Mitochondrial Ferritin antibody [EPR1797] (AB124889)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.