JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB305144

Anti-Mitochondrial Ferritin antibody [EPR25733-57] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Mitochondrial Ferritin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

FTMT

1 Images
Sandwich ELISA - Anti-Mitochondrial Ferritin antibody [EPR25733-57] - BSA and Azide free (Capture) (AB305144)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Mitochondrial Ferritin antibody [EPR25733-57] - BSA and Azide free (Capture) (AB305144)

Sandwich ELISA of ab305086 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25733-57

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitochondrial ferritin also known as ferritin 23 and abbreviated as FtMt is a protein that is part of the ferritin family. This mitochondrial protein displays a distinct structure with a molecular mass of approximately 150 kDa. It predominantly localizes in the mitochondria of cells where it serves an important role in iron storage. Unlike cytosolic ferritin which consists of H and L subunits mitochondrial ferritin arises as a homopolymer composed of 24 identical subunits. It is considered essential for maintaining mitochondrial iron homeostasis.
Biological function summary

Mitochondrial ferritin contributes to iron storage within mitochondria safeguarding cells from iron-mediated oxidative stress. It operates independently and does not incorporate into larger complexes like cytosolic ferritin does. Mitochondrial ferritin sequesters iron in a mineral form reducing the production of reactive oxygen species (ROS) that can damage cellular components. Its expression is particularly high in iron-rich tissues such as the testis and neurons suggesting a significance in tissues with active iron metabolism.

Pathways

Mitochondrial ferritin participates in iron metabolism and oxidative stress response. It affects the interaction with proteins like cytosolic ferritin and the transferrin receptor which are components of iron homeostasis pathways. Mitochondrial ferritin serves as a protective element by regulating iron storage within the mitochondria linking it to pathways that modulate oxidative stress. This interplay maintains cellular iron levels and prevents toxicity.

Mitochondrial ferritin's imbalance associates with neurodegenerative diseases and anemia. Neurodegenerative conditions such as Friedreich's ataxia reveal alterations in iron homeostasis where mitochondrial ferritin's role becomes apparent due to its protective function against mitochondrial damage. In anemia abnormal iron management results in either iron overload or deficiency where ferritin and transferrin receptor also play important roles. Understanding mitochondrial ferritin's function helps elucidate its contributions to the pathology of these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form (PubMed : 11323407, PubMed : 15201052). Important for iron homeostasis (PubMed : 11323407, PubMed : 15201052). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed : 11323407, PubMed : 15201052).
See full target information FTMT
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB83366

Iron Assay Kit (Colorimetric)

Biochemical assay - Iron Assay Kit (Colorimetric) (AB83366)

  • 4
  • 3
Primary Antibodies

AB214039

Anti-Transferrin Receptor antibody [EPR20584]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin Receptor antibody [EPR20584] (AB214039)

  • 5
  • 1
Primary Antibodies

AB75973

Anti-Ferritin heavy chain antibody [EPR3004Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973)

  • 4
  • 7
Primary Antibodies

AB189491

Anti-Heme Oxygenase 1 antibody [EPR18161-128]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com