Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunohistochemical analysis of paraffin-embedded human colon tissue labelling Mitochondrial ribosomal protein L11 using ab133789 (unpurified) at 1/250 dilution

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunofluorescent staining of Mitochondrial ribosomal protein L11 in HEPG2 cells using ab133789 (unpurified) at 1/250 dilution

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Mitochondrial ribosomal protein L11 using ab133789 (unpurified) at 1/250 dilution

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Mitochondrial ribosomal protein L11 with purified ab133789 at 1/120 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Mitochondrial ribosomal protein L11 with purified ab133789 at 1 : 100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Mitochondrial ribosomal protein L11 with purified ab133789 at 1 : 200 dilution (6.2 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Overlay histogram showing HepG2 cells stained with unpurified ab133789 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133789, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

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Western blot - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

All lanes:

Western blot - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (ab133789) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

LNCaP cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 21 kDa

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• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal heart tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal lung tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal stomach tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

Immunohistochemical analysis using ab133789 (unpurified) showing positive staining in Normal colon tissue.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

All lanes:

Western blot - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (ab133789) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (AB133789)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.