Rabbit Recombinant Monoclonal Mitochondrial ribosomal protein L11 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (AB248654), expandable thumbnail](https://content.abcam.com/products/images/mitochondrial-ribosomal-protein-l11-antibody-epr9111b-bsa-and-azide-free-ab248654--flow-cytometry-intracellular-img37907.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (AB248654)")
![Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (AB248654), expandable thumbnail](https://content.abcam.com/products/images/mitochondrial-ribosomal-protein-l11-antibody-epr9111b-bsa-and-azide-free-ab248654--immunocytochemistry-immunofluorescence-img66318.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (AB248654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (AB248654), expandable thumbnail](https://content.abcam.com/products/images/mitochondrial-ribosomal-protein-l11-antibody-epr9111b-bsa-and-azide-free-ab248654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108023.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (AB248654)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Alternative names
CGI-113, MRPL11, Large ribosomal subunit protein uL11m, L11mt, MRP-L11
Recommended products
Rabbit Recombinant Monoclonal Mitochondrial ribosomal protein L11 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9111(B)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 8.1 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248654 is the carrier-free version of Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] ab133789.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Mitochondrial ribosomal protein L11 often called MRPL11 forms an important part of the mitochondrial ribosome or mitoribosome which plays an important role in protein synthesis within the mitochondria. MRPL11 has a mass of approximately 21.3 kDa and is expressed in tissues with high metabolic activity such as the heart and skeletal muscles. It differs from proteins found in the cytosolic ribosomes due to variations in protein to rRNA ratio and the presence of specific mitochondrial sequences.
- Biological function summary
This protein contributes to the formation and functional stability of the large subunit of the mitochondrial ribosome. MRPL11 is an integral part of the mitoribosomal complex and it collaborates closely with mitochondrial ribosome protein L10 and L12. The assembly of these proteins ensures efficient mitochondrial translation which subsequently impacts mitochondrial energy production and apoptotic signaling.
- Pathways
MRPL11 is deeply embedded in mitochondrial translation and oxidative phosphorylation pathways. These pathways are essential for cellular energy production and metabolism. MRPL11 interacts with other mitochondrial proteins such as mRNA encoders and enzymes in the electron transport chain. It ensures correct assembly and function of the mitochondrial machinery influencing energy demands through the connection to translation regulation and metabolic balance.
- Associated diseases and disorders
MRPL11 is associated with mitochondrial disorders like mitochondrial myopathy and neurodegenerative diseases. Mutations or dysregulations in MRPL11 can impair mitochondrial translation leading to compromised energy metabolism. Its dysfunction can involve other mitochondrial proteins such as those forming the electron transport chain or involved in cardiolipin metabolism contributing to the disease mechanisms. Understanding MRPL11's role offers insights into therapeutic targets for disease intervention in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654), expandable thumbnail](https://content.abcam.com/products/images/mitochondrial-ribosomal-protein-l11-antibody-epr9111b-bsa-and-azide-free-ab248654--flow-cytometry-intracellular-img37907.jpg "Flow Cytometry (Intracellular) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654)")
Flow Cytometry (Intracellular) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Mitochondrial ribosomal protein L11 with purified Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] ab133789 at 1/120 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] ab133789).
![Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654), expandable thumbnail](https://content.abcam.com/products/images/mitochondrial-ribosomal-protein-l11-antibody-epr9111b-bsa-and-azide-free-ab248654--immunocytochemistry-immunofluorescence-img66318.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654)")
Immunocytochemistry/ Immunofluorescence - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654)
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Mitochondrial ribosomal protein L11 with purified Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] ab133789 at 1/100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] ab133789).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654), expandable thumbnail](https://content.abcam.com/products/images/mitochondrial-ribosomal-protein-l11-antibody-epr9111b-bsa-and-azide-free-ab248654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108023.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] - BSA and Azide free (ab248654)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Mitochondrial ribosomal protein L11 with purified Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] ab133789 at 1/200 dilution (6.2 μg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] ab133789).
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