Rabbit Polyclonal Mitofilin antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IMMT aa 50-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IMMT aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q16891
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/2000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/5000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
Associated Products
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Target data
Function
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed:22114354, PubMed:25781180, PubMed:32567732, PubMed:33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180, PubMed:32567732, PubMed:33130824).
Alternative names
HMP, MIC60, MINOS2, PIG4, PIG52, IMMT, MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein, Mitochondrial inner membrane protein, Mitofilin, p87/89
Recommended products
Rabbit Polyclonal Mitofilin antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IMMT aa 50-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IMMT aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q16891
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Mitofilin also known as mitofilin or MIM is a protein with a mass of approximately 88 kDa. This protein is integral to the inner mitochondrial membrane. Mitofilin is found in various tissues with high expression in the heart and skeletal muscle indicating its significant role in energy-demanding tissues. It is the central component of the mitochondrial inner membrane organizing system (MINOS) which plays a fundamental role in maintaining the mitochondrial architecture.
- Biological function summary
Within the mitochondrial environment mitofilin contributes to maintaining cristae morphology which is essential for optimal mitochondrial respiration and energy production. As part of the MINOS complex mitofilin interacts with other proteins such as Mic60 and Mic10 stabilizing the structure of crista junctions. This process ensures efficient electron transport chain function ultimately supporting ATP synthesis. Without effective shaping of the cristae cellular energy metabolism deteriorates.
- Pathways
Research has highlighted the importance of mitofilin in apoptosis and bioenergetics pathways. It interacts with proteins like OPA1 a dynamin-related GTPase important for mitochondrial fusion and energy metabolism. Additionally mitofilin influences the release of cytochrome c an important step in the initiation of apoptosis. Proper functioning of these pathways is critical for cellular balance and survival showing mitofilin’s broad impact on cellular processes.
- Associated diseases and disorders
Mitofilin has links to mitochondrial myopathies and cardiomyopathies. These disorders stem from impaired mitochondrial function significantly affecting muscle tissues and heart performance. Disruption in mitofilin expression or function can lead to compromised energy production contributing to these conditions. Furthermore mitofilin has associations with proteins like Drp1 involved in mitochondrial dynamics and this relationship can influence the progression and severity of mitochondrial-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-Mitofilin antibody (ab229653)
All lanes: Western blot - Anti-Mitofilin antibody (ab229653) at 1/1000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (ab229653)
Paraffin-embedded human glioma cancer tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (ab229653)
Paraffin-embedded human kidney tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-Mitofilin antibody (ab229653)
Mitofilin was immunoprecipitated from 500 μg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 8 μg ab229653.
Lane 1: Rabbit monoclonal IgG (1 μg) IP in HepG2 whole cell lysate.
Lane 2: ab229653 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 μg.
For western blotting, an HRP-conjugated Protein G antibody was used as the Secondary antibody at 1/2000 dilution.
All lanes: Immunoprecipitation - Anti-Mitofilin antibody (ab229653)
Predicted band size: 84 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody (ab229653)
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mitofilin (green) using ab229653 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
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