AB229653

Anti-Mitofilin antibody

Name: Anti-Mitofilin antibody (ab229653) | Abcam
Brand: Abcam Limited
SKU: AB229653

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(1 Publication)

Rabbit Polyclonal Mitofilin antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IMMT aa 50-250.

View Alternative Names

HMP, MIC60, MINOS2, PIG4, PIG52, IMMT, MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein, Mitochondrial inner membrane protein, Mitofilin, p87/89

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (AB229653)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (AB229653)

Paraffin-embedded human kidney tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (AB229653)

Paraffin-embedded human glioma cancer tissue stained for Mitofilin using ab229653 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody (AB229653)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody (AB229653)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Mitofilin (green) using ab229653 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® congugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Immunoprecipitation - Anti-Mitofilin antibody (AB229653)

Mitofilin was immunoprecipitated from 500 μg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with 8 μg ab229653.

Lane 1 : Rabbit monoclonal IgG (1 μg) IP in HepG2 whole cell lysate.

Lane 2 : ab229653 IP in HepG2 whole cell lysate.

Lane 3 : HepG2 whole cell lysate 10 μg.

For western blotting, an HRP-conjugated Protein G antibody was used as the Secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-Mitofilin antibody (ab229653)

Predicted band size: 84 kDa

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Supplier Data

Western blot - Anti-Mitofilin antibody (AB229653)

All lanes:

Western blot - Anti-Mitofilin antibody (ab229653) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IMMT aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q16891

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitofilin also known as mitofilin or MIM is a protein with a mass of approximately 88 kDa. This protein is integral to the inner mitochondrial membrane. Mitofilin is found in various tissues with high expression in the heart and skeletal muscle indicating its significant role in energy-demanding tissues. It is the central component of the mitochondrial inner membrane organizing system (MINOS) which plays a fundamental role in maintaining the mitochondrial architecture.

Biological function summary

Within the mitochondrial environment mitofilin contributes to maintaining cristae morphology which is essential for optimal mitochondrial respiration and energy production. As part of the MINOS complex mitofilin interacts with other proteins such as Mic60 and Mic10 stabilizing the structure of crista junctions. This process ensures efficient electron transport chain function ultimately supporting ATP synthesis. Without effective shaping of the cristae cellular energy metabolism deteriorates.

Pathways

Research has highlighted the importance of mitofilin in apoptosis and bioenergetics pathways. It interacts with proteins like OPA1 a dynamin-related GTPase important for mitochondrial fusion and energy metabolism. Additionally mitofilin influences the release of cytochrome c an important step in the initiation of apoptosis. Proper functioning of these pathways is critical for cellular balance and survival showing mitofilin's broad impact on cellular processes.

Mitofilin has links to mitochondrial myopathies and cardiomyopathies. These disorders stem from impaired mitochondrial function significantly affecting muscle tissues and heart performance. Disruption in mitofilin expression or function can lead to compromised energy production contributing to these conditions. Furthermore mitofilin has associations with proteins like Drp1 involved in mitochondrial dynamics and this relationship can influence the progression and severity of mitochondrial-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed : 22114354, PubMed : 25781180, PubMed : 32567732, PubMed : 33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed : 22114354, PubMed : 25781180, PubMed : 32567732, PubMed : 33130824).

See full target information IMMT

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Current biology : CB 33:3805-3820.e7 PubMed37586372

2023

Optineurin tunes outside-in signaling to regulate lysosome biogenesis and phagocytic clearance in the retina.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xuan Tan,Colin J Germer,Thushara Thamban,Nilsa La Cunza,Aparna Lakkaraju

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mitofilin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (AB229653)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody (AB229653)

Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody (AB229653)

Immunoprecipitation - Anti-Mitofilin antibody (AB229653)

Western blot - Anti-Mitofilin antibody (AB229653)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise