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Rabbit Recombinant Monoclonal Mitofilin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (AB245764), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (AB245764), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (AB245764), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (AB245764), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (AB245764), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

7 products for Alternative Product

8 products for Alternative Version

Target data

Function

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed:22114354, PubMed:25781180, PubMed:32567732, PubMed:33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180, PubMed:32567732, PubMed:33130824).

Alternative names

HMP, MIC60, MINOS2, PIG4, PIG52, IMMT, MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein, Mitochondrial inner membrane protein, Mitofilin, p87/89

Recommended products

Rabbit Recombinant Monoclonal Mitofilin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8749
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab245764 is the carrier-free version of Anti-Mitofilin antibody [EPR8749] ab137057.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Mitofilin also known as mitofilin or MIM is a protein with a mass of approximately 88 kDa. This protein is integral to the inner mitochondrial membrane. Mitofilin is found in various tissues with high expression in the heart and skeletal muscle indicating its significant role in energy-demanding tissues. It is the central component of the mitochondrial inner membrane organizing system (MINOS) which plays a fundamental role in maintaining the mitochondrial architecture.

Biological function summary

Within the mitochondrial environment mitofilin contributes to maintaining cristae morphology which is essential for optimal mitochondrial respiration and energy production. As part of the MINOS complex mitofilin interacts with other proteins such as Mic60 and Mic10 stabilizing the structure of crista junctions. This process ensures efficient electron transport chain function ultimately supporting ATP synthesis. Without effective shaping of the cristae cellular energy metabolism deteriorates.

Pathways

Research has highlighted the importance of mitofilin in apoptosis and bioenergetics pathways. It interacts with proteins like OPA1 a dynamin-related GTPase important for mitochondrial fusion and energy metabolism. Additionally mitofilin influences the release of cytochrome c an important step in the initiation of apoptosis. Proper functioning of these pathways is critical for cellular balance and survival showing mitofilin’s broad impact on cellular processes.

Associated diseases and disorders

Mitofilin has links to mitochondrial myopathies and cardiomyopathies. These disorders stem from impaired mitochondrial function significantly affecting muscle tissues and heart performance. Disruption in mitofilin expression or function can lead to compromised energy production contributing to these conditions. Furthermore mitofilin has associations with proteins like Drp1 involved in mitochondrial dynamics and this relationship can influence the progression and severity of mitochondrial-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Western blot - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057).

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-Mitofilin antibody [EPR8749] ab137057 observed at 84 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    Anti-Mitofilin antibody [EPR8749] ab137057 was shown to specifically react with IMMT in wild-type HAP1 cells as signal was lost in IMMT knockout cells. Wild-type and IMMT knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Anti-Mitofilin antibody [EPR8749] ab137057 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: IMMT knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HepG2 whole cell lysate at 20 µg

    Lane 4: Human Heart whole cell lysate at 20 µg

    Predicted band size: 84 kDa

    Observed band size: 84 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid cancer tissue sections labeling Mitofilin with purified Anti-Mitofilin antibody [EPR8749] ab137057 at 1/250 dilution (0.508 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular cancer tissue sections labeling Mitofilin with purified Anti-Mitofilin antibody [EPR8749] ab137057 at 1/250 dilution (0.508 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057).

  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Mitofilin with purified Anti-Mitofilin antibody [EPR8749] ab137057 at 1/100 dilution (1.3 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Mitofilin with Anti-Mitofilin antibody [EPR8749] ab137057 at 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; glycerol and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunohistochemical analysis of paraffin embedded Human testis tissue labelling Mitofilin with Anti-Mitofilin antibody [EPR8749] ab137057 at 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; glycerol and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057)

  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Immunofluorescent staining of HeLa cells labelling Mitofilin with Anti-Mitofilin antibody [EPR8749] ab137057 at 1/250 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; glycerol and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057)

  • Flow Cytometry (Intracellular) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Mitofilin antibody [EPR8749] - BSA and Azide free (ab245764)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Mitofilin with purified Anti-Mitofilin antibody [EPR8749] ab137057 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Mitofilin antibody [EPR8749] ab137057).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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