Anti-Mitofusin 1 antibody [11E91H12]

• WB

Unknown

Western blot - Anti-Mitofusin 1 antibody [11E91H12] (AB126575)

Lower panel : Anti-Actin antibody (ab3280) at 0.5µg/mL.

All lanes:

Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/mL

Lane 1:

H4IIE Rat cell lysate at 40 µg

Lane 2:

NIH 3T3 Mouse cell lysate at 40 µg

Lane 3:

Mouse embryonic fibroblast cell line, wild-type at 40 µg

Lane 4:

Mouse embryonic fibroblast cell line, Mitofusin 1-deficient at 40 µg

Lane 5:

Mouse embryonic fibroblast cell line, Mitofusin 2-deficient at 40 µg

Secondary

All lanes:

Goat-anti-Mouse HRP at 1/3000 dilution

Predicted band size: 84 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 1 antibody [11E91H12] (AB126575)

ab126575 at 5 µg/ml (2 hrs) staining Mitofusin 1 in paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min) Mouse embryonic fibroblast cells by Immunofluorescence. Secondary : 1/2000 AlexaFluor® 488 goat anti-mouse IgG (H+L). DAPI was used to stain the cell nuclei (blue). Abcam Blocking Buffer (ab126587) was used throughout.

• WB

Unknown

Western blot - Anti-Mitofusin 1 antibody [11E91H12] (AB126575)

Left panel : 4% milk was used as the blocking agent throughout the western blot. Right panel : 4% milk was used for the initial block and for the secondary antibody incubation, but ab126575 was diluted in Abcam’s Blocking Buffer (ab126587). Note that Anti-Mitofusin 1 antibody is not compatible with dilution in milk blocking buffer.

All lanes:

Western blot - Anti-Mitofusin 1 antibody [11E91H12] (ab126575) at 5 µg/mL

Lane 1:

H4IIE Rat cell lysate at 40 µg

Lane 2:

Mouse embryonic fibroblast cell line, wild-type at 40 µg

Lane 3:

Mouse embryonic fibroblast cell line, Mitofusin 1-deficient at 40 µg

Lane 4:

Mouse embryonic fibroblast cell line, Mitofusin 2-deficient at 40 µg

Secondary

All lanes:

Goat-anti-Mouse HRP at 1/3000 dilution

Predicted band size: 84 kDa

false