AB221661

Anti-Mitofusin 1 antibody [EPR21953-74]

Name: Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) | Abcam
Brand: Abcam Limited
SKU: AB221661
Price: 200 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
20ul selling size
What is this?

(1 Review)

(41 Publications)

Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) is a rabbit monoclonal antibody detecting Mitofusin 1 in Western Blot, IP. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Mitofusin-1, Transmembrane GTPase MFN1, Mfn1

3 Images

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Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] (AB221661)

Mitofusin 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab221661 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221661 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2 : ab221661 IP in NIH/3T3 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab221661 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661)

Predicted band size: 84 kDa

Observed band size: 84 kDa

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Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (AB221661)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1-3 : 3 minutes. Lane 4 : 81 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 25574749).

All lanes:

Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg

Lane 2:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Lane 4:

Rat heart lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Unknown

Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (AB221661)

Blocking and dilution buffer : 5% NFDM/TBST.

Eposure times.

Lane 1 : 3 minutes. Lanes 2 & 3 : 48 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 25574749).

All lanes:

Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Lane 3:

Mouse heart lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Carrier free

Anti-Mitofusin 1 antibody [EPR21953-74] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21953-74

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Mitofusin 1 antibody [EPR21953-74] (ab221661) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Mouse, Rat samples.

What is the molecular weight of Mitofusin 1?
Anti-Mitofusin 1 [EPR21953-74] (ab221661) specifically detects a band for Mitofusin 1 (UniProt: Q811U4) at a molecular weight of 84kDa.

Trusted by the scientific community
Anti-Mitofusin 1 [EPR21953-74] (ab221661) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21953-74] also available for your convenience: ab221661, Carrier free - ab254198

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitofusin 1 (MFN1) also known as mfn1 is a GTPase protein involved in mitochondrial fusion with a molecular weight of approximately 84 kDa. This protein is expressed in various tissues most notably in skeletal muscle cardiac muscle and the nervous system. Mitofusin 1 plays a significant role in maintaining mitochondrial structure and function by facilitating the fusion of outer mitochondrial membranes which is essential for normal mitochondrial dynamics.

Biological function summary

Mitofusin 1 is involved in processes beyond just mitochondrial fusion. It participates in mitochondrial quality control and bioenergetics helping to optimize the distribution of mitochondrial DNA and components across the mitochondrial network. Mitofusin 1 is also part of a complex that involves Mitofusin 2 (MFN2). Together these proteins regulate interactions between mitochondria and other organelles contributing to cellular homeostasis and adaptation to metabolic demands.

Pathways

Mitofusin 1 plays a role in the mitochondrial fusion and fission pathway which is critical for mitochondrial and cellular health. It interacts closely with other proteins like OPA1 (optic atrophy 1) an important regulator in the fusion process. Moreover Mitofusin 1 is integrated into the apoptosis pathway where it influences cell survival and death. Its interaction with Bak and Bax proteins which are involved in apoptotic signaling highlights the role of Mitofusin 1 in balancing cell fate decisions.

Mutations or dysregulation of Mitofusin 1 have associations with neurodegenerative diseases and cardiomyopathies. For instance defects in Mitofusin 1 are linked to Charcot-Marie-Tooth disease type 2A a disorder affecting peripheral nerves. Additionally disturbances in Mitofusin 1 function have implications for heart health particularly in relation to heart failure where proper mitochondrial dynamics are disrupted. Through these conditions Mitofusin 1 also interacts with associated proteins like Mitofusin 2 reflecting shared roles in the pathology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed : 12527753, PubMed : 15297672, PubMed : 23921378, PubMed : 24513856). Membrane clustering requires GTPase activity (By similarity). It may involve a major rearrangement of the coiled coil domains (PubMed : 15297672). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed : 12527753). Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity (By similarity).

See full target information Mfn1

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Publications (41)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0256 PubMed40980288

2025

Decreasing Lamin A Triggers Cell Fate Transitions through Heterochromatin-Nuclear Periphery Detethering.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Sun,Yafan Xie,Zhaoyan Zuo,Jian Liu,Jiaqi Yang,Iqra Ali,Qin Peng,Juhui Qiu

Frontiers in pharmacology 16:1566674 PubMed40978492

2025

Hyperoside alleviates myocardial ischemia-reperfusion injury in heart transplantation by promoting mitochondrial fusion via activating the Stat3-Tom70-Opa1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jincheng Hou,Hongwen Lan,Chenghao Li,Zihao Wang,Qiang Zheng,Kan Wang,Tixiusi Xiong,Yixuan Wang,Jiawei Shi,Nianguo Dong

Journal of cell communication and signaling 19:e70044 PubMed40917114

2025

Trans-Coumaryl acetate mediates GRK5/NF-κB/Nrf2 signaling axis to ameliorate septic acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Yugang Deng,Kunyang Lei,Yaqi Li,Siwei Ma

Cardiovascular diabetology 24:331 PubMed40804395

2025

Mitochondrial ultrastructural pathology in diabetic cardiomyopathy: integrated analysis via scanning electron microscopy and 3D visualization imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Linghao Dai,Haowei Liang,Jiayu He,Jiayi Zhou,Yang Guan,Hui Wang

Frontiers in pharmacology 16:1560608 PubMed40356981

2025

Irisin attenuates cardiac injury and improves prognosis in rats with hemorrhagic shock by maintaining mitochondrial homeostasis via the AMPK/Drp1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zhang,Yufang Zhang,Xiaofang Zou,Jiake Li,Yunfei Chi,Hailiang Bai,Bin Wei,Huiting Yun,Quanxi Zhang,Weihua Cao,Haiyan Liu,Hongjie Duan

Respiratory research 26:159 PubMed40275330

2025

ETS2 aggravate allergic airway inflammation by regulating ANT2-mediated cytosolic mitochondrial DsRNA levels.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Jiang,Yaona Jiang,Ran Dong,Chang-Yong Fu

Scientific reports 15:11698 PubMed40188200

2025

Exercise enhances cardiomyocyte mitochondrial homeostasis to alleviate left ventricular dysfunction in pressure overload induced remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Ma,Yanling Cen,Weiwei Xun,Caiying Mou,Junwen Yu,Yarui Hu,Chen Liu,Jun Sun,Rui Bi,Yanli Qiu,Mingchao Ding,Li Jin

Cell biology and toxicology 41:7 PubMed39707117

2024

METTL3, m6A modification, and EGR1: interplay affecting myocardial I/R injury outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huang,Xun Zhang,Shi-Xiong Wu,Qing Chang,Zhi-Kun Zheng,Jing Xu

Stem cell research & therapy 15:412 PubMed39523354

2024

Pretreatment with growth differentiation factor 15 augments cardioprotection by mesenchymal stem cells in myocardial infarction by improving their survival.

Applications

Unspecified application

Species

Unspecified reactive species

Xinran Huang,Xiaoting Liang,Qian Han,Ying Shen,Jiaqi Chen,Ziqi Li,Jie Qiu,Xiaoyan Gao,Yimei Hong,Fang Lin,Weifeng Li,Xin Li,Yuelin Zhang

Redox report : communications in free radical research 29:2404794 PubMed39314036

2024

WTAP-mediated mA modification of TRIM22 promotes diabetic nephropathy by inducing mitochondrial dysfunction via ubiquitination of OPA1.

Applications

Unspecified application

Species

Unspecified reactive species

Zeng Zhang,Fengzhu Zhou,Min Lu,Duanchun Zhang,Xinyi Zhang,Siyu Xu,Yanming He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mitofusin 1 antibody [EPR21953-74]

Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] (AB221661)

Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (AB221661)

Western blot - Anti-Mitofusin 1 antibody [EPR21953-74] (AB221661)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (41)

Product promise