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AB254198

Anti-Mitofusin 1 antibody [EPR21953-74] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Mitofusin 1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Mitofusin-1, Transmembrane GTPase MFN1, Mfn1

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Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] - BSA and Azide free (AB254198)
  • IP

Unknown

Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] - BSA and Azide free (AB254198)

Mitofusin 1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab221661 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221661 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2 : ab221661 IP in NIH/3T3 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab221661 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221661).

All lanes:

Immunoprecipitation - Anti-Mitofusin 1 antibody [EPR21953-74] (<a href='/en-us/products/primary-antibodies/mitofusin-1-antibody-epr21953-74-ab221661'>ab221661</a>)

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21953-74

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab254198 is the carrier-free version of ab221661.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitofusin 1 (MFN1) also known as mfn1 is a GTPase protein involved in mitochondrial fusion with a molecular weight of approximately 84 kDa. This protein is expressed in various tissues most notably in skeletal muscle cardiac muscle and the nervous system. Mitofusin 1 plays a significant role in maintaining mitochondrial structure and function by facilitating the fusion of outer mitochondrial membranes which is essential for normal mitochondrial dynamics.
Biological function summary

Mitofusin 1 is involved in processes beyond just mitochondrial fusion. It participates in mitochondrial quality control and bioenergetics helping to optimize the distribution of mitochondrial DNA and components across the mitochondrial network. Mitofusin 1 is also part of a complex that involves Mitofusin 2 (MFN2). Together these proteins regulate interactions between mitochondria and other organelles contributing to cellular homeostasis and adaptation to metabolic demands.

Pathways

Mitofusin 1 plays a role in the mitochondrial fusion and fission pathway which is critical for mitochondrial and cellular health. It interacts closely with other proteins like OPA1 (optic atrophy 1) an important regulator in the fusion process. Moreover Mitofusin 1 is integrated into the apoptosis pathway where it influences cell survival and death. Its interaction with Bak and Bax proteins which are involved in apoptotic signaling highlights the role of Mitofusin 1 in balancing cell fate decisions.

Mutations or dysregulation of Mitofusin 1 have associations with neurodegenerative diseases and cardiomyopathies. For instance defects in Mitofusin 1 are linked to Charcot-Marie-Tooth disease type 2A a disorder affecting peripheral nerves. Additionally disturbances in Mitofusin 1 function have implications for heart health particularly in relation to heart failure where proper mitochondrial dynamics are disrupted. Through these conditions Mitofusin 1 also interacts with associated proteins like Mitofusin 2 reflecting shared roles in the pathology of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed : 12527753, PubMed : 15297672, PubMed : 23921378, PubMed : 24513856). Membrane clustering requires GTPase activity (By similarity). It may involve a major rearrangement of the coiled coil domains (PubMed : 15297672). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed : 12527753). Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity (By similarity).
See full target information Mfn1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 14:7219-7240 PubMed39629129

2024

Gut microbiota depletion and FXR inhibition exacerbates zonal hepatotoxicity of sunitinib.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhao,Yingmei Lu,Jingyi Duan,Dan Du,Qianlun Pu,Fei Li
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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