AB191853

Anti-Mitofusin 1 antibody - N-terminal

Name: Anti-Mitofusin 1 antibody - N-terminal (ab191853) | Abcam
Brand: Abcam Limited
SKU: AB191853
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal Mitofusin 1 antibody. N-terminal. Suitable for ICC, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MFN1.

View Alternative Names

Mitofusin-1, Fzo homolog, Transmembrane GTPase MFN1, MFN1

2 Images

Supplier Data

Immunocytochemistry - Anti-Mitofusin 1 antibody - N-terminal (AB191853)

Immunocytochemistry analysis of A431 cells, labeling Mitofusin 1 using ab191853 at 5 μg/mL.

Supplier Data

Western blot - Anti-Mitofusin 1 antibody - N-terminal (AB191853)

All lanes:

Western blot - Anti-Mitofusin 1 antibody - N-terminal (ab191853) at 1 µg/mL

All lanes:

A431 cell lysate at 15 µg

Predicted band size: 84 kDa

Observed band size: 83 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human MFN1. The exact immunogen used to generate this antibody is proprietary information.

Q8IWA4

Specificity

ab191853 is predicted to not cross-react with Mitofusin 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitofusin 1 (MFN1) also known as mfn1 is a GTPase protein involved in mitochondrial fusion with a molecular weight of approximately 84 kDa. This protein is expressed in various tissues most notably in skeletal muscle cardiac muscle and the nervous system. Mitofusin 1 plays a significant role in maintaining mitochondrial structure and function by facilitating the fusion of outer mitochondrial membranes which is essential for normal mitochondrial dynamics.

Biological function summary

Mitofusin 1 is involved in processes beyond just mitochondrial fusion. It participates in mitochondrial quality control and bioenergetics helping to optimize the distribution of mitochondrial DNA and components across the mitochondrial network. Mitofusin 1 is also part of a complex that involves Mitofusin 2 (MFN2). Together these proteins regulate interactions between mitochondria and other organelles contributing to cellular homeostasis and adaptation to metabolic demands.

Pathways

Mitofusin 1 plays a role in the mitochondrial fusion and fission pathway which is critical for mitochondrial and cellular health. It interacts closely with other proteins like OPA1 (optic atrophy 1) an important regulator in the fusion process. Moreover Mitofusin 1 is integrated into the apoptosis pathway where it influences cell survival and death. Its interaction with Bak and Bax proteins which are involved in apoptotic signaling highlights the role of Mitofusin 1 in balancing cell fate decisions.

Mutations or dysregulation of Mitofusin 1 have associations with neurodegenerative diseases and cardiomyopathies. For instance defects in Mitofusin 1 are linked to Charcot-Marie-Tooth disease type 2A a disorder affecting peripheral nerves. Additionally disturbances in Mitofusin 1 function have implications for heart health particularly in relation to heart failure where proper mitochondrial dynamics are disrupted. Through these conditions Mitofusin 1 also interacts with associated proteins like Mitofusin 2 reflecting shared roles in the pathology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed : 12475957, PubMed : 12759376, PubMed : 27920125, PubMed : 28114303). Membrane clustering requires GTPase activity (PubMed : 27920125). It may involve a major rearrangement of the coiled coil domains (PubMed : 27920125, PubMed : 28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed : 12475957, PubMed : 12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed : 12759376). Has low GTPase activity (PubMed : 27920125, PubMed : 28114303).

See full target information MFN1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 17:188 PubMed39289738

2024

Mechanism study of YangJing ZhongYu decoction on regulating mitochondrial dynamics of ovarian granular cells and improving diminished ovarian reserve.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Jilin Kuang

Nature communications 14:6900 PubMed37903764

2023

DIAPH1-MFN2 interaction regulates mitochondria-SR/ER contact and modulates ischemic/hypoxic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Gautham Yepuri,Lisa M Ramirez,Gregory G Theophall,Sergei V Reverdatto,Nosirudeen Quadri,Syed Nurul Hasan,Lei Bu,Devi Thiagarajan,Robin Wilson,Raquel López Díez,Paul F Gugger,Kaamashri Mangar,Navneet Narula,Stuart D Katz,Boyan Zhou,Huilin Li,Aleksandr B Stotland,Roberta A Gottlieb,Ann Marie Schmidt,Alexander Shekhtman,Ravichandran Ramasamy

Redox biology 64:102786 PubMed37348156

2023

Metformin inhibits methylglyoxal-induced retinal pigment epithelial cell death and retinopathy via AMPK-dependent mechanisms: Reversing mitochondrial dysfunction and upregulating glyoxalase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Ponarulselvam Sekar,George Hsiao,Shu-Hao Hsu,Duen-Yi Huang,Wan-Wan Lin,Chi-Ming Chan

Cells 11: PubMed36078067

2022

A Combination Therapy of Urolithin A+EGCG Has Stronger Protective Effects than Single Drug Urolithin A in a Humanized Amyloid Beta Knockin Mice for Late-Onset Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sudhir Kshirsagar,Rainier Vladlen Alvir,Jangampalli Adi Pradeepkiran,Ashly Hindle,Murali Vijayan,Bhagavathi Ramasubramaniam,Subodh Kumar,Arubala P Reddy,P Hemachandra Reddy

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Mitofusin 1 antibody - N-terminal

Immunocytochemistry - Anti-Mitofusin 1 antibody - N-terminal (AB191853)

Western blot - Anti-Mitofusin 1 antibody - N-terminal (AB191853)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise