Anti-Mitofusin 2 antibody [6A8]

• WB

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Western blot - Anti-Mitofusin 2 antibody [6A8] (AB56889)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Mitofusin 2 antibody [6A8] (ab56889) at 1 µg/mL

All lanes:

HeLa cell lysate at 25 µg

Predicted band size: 86 kDa

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• Flow Cyt

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Flow Cytometry - Anti-Mitofusin 2 antibody [6A8] (AB56889)

Overlay histogram showing HEK293 cells stained with ab56889 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56889, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [6A8] (AB56889)

Mitofusin 2 antibody (ab56889) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.

This image was generated using the ascites version of the product.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [6A8] (AB56889)

ab56889 staining mitofusin2 in MEF1 cells treated with valinomycin from Streptomyces fulvissimus (ab120852), by ICC/IF. Decrease in mitofusin2 expression with increased concentration of withaferin valinomycin from Streptomyces fulvissimus, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120852 (valinomycin from Streptomyces fulvissimus) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab56889 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight^® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

This image was generated using the ascites version of the product.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [6A8] (AB56889)

ab56889 staining mitofusin 2 in MEF1 cells treated with nigericin Na⁺ salt (ab120494), by ICC/IF. Decrease in mitofusin 2 expression correlates with increased concentration of nigericin Na⁺ salt, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120494 (nigericin Na⁺ salt) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab56889 (10 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight^® 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

This image was generated using the ascites version of the product.