Anti-Mitofusin 2 antibody

• WB

Unknown

Western blot - Anti-Mitofusin 2 antibody (AB50843)

All lanes:

Western blot - Anti-Mitofusin 2 antibody (ab50843) at 0.25 µg/mL

Lane 1:

Rat brain mitochondria

Lane 2:

Mouse brain mitochondria

Secondary

All lanes:

Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.

Predicted band size: 86 kDa

Observed band size: 86 kDa

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• WB

CiteAb

Western blot - Anti-Mitofusin 2 antibody (AB50843)

Mitofusin 2 western blot using anti-Mitofusin 2 antibody ab50843. Publication image and figure legend from Ruiz, L., Salazar, C., et al., 2015, Oxid Med Cell Longev, PubMed 26106459.

ab50843 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab50843 please see the product overview.

Effect of quercetin treatment on the expression of PGC-1α, Mitofusin 2, VDAC, and protein oxidation. (a) Detection of carbonyl groups was performed with the OxyBlot Protein Oxidation Detection Kit. (c) Densitometry quantification of carbonyl groups was made with the ImageJ software. Carbonylation of proteins was normalized by Ponceau staining and Complex V (CV) expression. (b) Expression of mitochondrial proteins. Protein expression of MFN2, PGC-1α, and VDAC1 was analyzed in heart isolated mitochondria from control and quercetin-treated mice. β-actin was used as a loading control. (d) Densitometry analysis. MFN2, PGC-1α, and VDAC1 expressions were normalized by β-actin expression. Each bar represents the mean ± SD, analyzed by two-sample t-test (P < 0.05). Control mice, n = 11; quercetin-treated mice, n = 9. Each bar represents the mean ± SD, analyzed by two-sample t-test (P < 0.05). Significant differences (*) were found between control and quercetin-treated mice. P < 0.05.

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