AB186317

Anti-Mitofusin 2 antibody [N153/5]

Name: Anti-Mitofusin 2 antibody [N153/5] (ab186317) | Abcam
Brand: Abcam Limited
SKU: AB186317

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(1 Publication)

Mouse Monoclonal Mitofusin 2 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Mfn2 aa 350-600.

View Alternative Names

Fzo1a, Mfn2, Mitofusin-2, Mitochondrial transmembrane GTPase FZO1A, Transmembrane GTPase MFN2, Protein HSG

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [N153/5] (AB186317)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [N153/5] (AB186317)

ab186317 staining Mitofusin 2 in the Human Neuroblastoma cell line (C) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with4% formaldehyde for 15 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. An ATTO 488-conjugated Goat anti-mouse polyclonal was used as the secondary antibody (1/100). Nucelus stained blue with DAPI (A), F-Actin stained red with Phalloidin Texas Red (B) and merged images (D)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [N153/5] (AB186317)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling Mitofusin 2 with ab186317 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Mitofusin 2 Antibody (D) Composite.

Supplier Data

Western blot - Anti-Mitofusin 2 antibody [N153/5] (AB186317)

All lanes:

Western blot - Anti-Mitofusin 2 antibody [N153/5] (ab186317) at 1/200 dilution

All lanes:

Rat brain membrane lysate at 15 µg

Predicted band size: 86 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N153/5

Isotype

IgG2a

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Mfn2 aa 350-600. The exact immunogen used to generate this antibody is proprietary information.

Q80U63

Specificity

ab186317 does not cross react with Mitofusin 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from S153-5 to N153/5, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mitofusin 2 also known as MFN2 is a protein involved in the regulation of mitochondrial fusion. The MFN2 molecular weight is roughly 86 kDa. It plays an important role in connecting and merging the outer membranes of mitochondria which is vital for maintaining mitochondrial function and integrity. Mitofusin 2 proteins are expressed in many tissues but they are abundantly present in energy-demanding tissues like skeletal muscle heart and the brain.

Biological function summary

Mitofusin 2 ensures the proper distribution of mitochondria within cells and regulates mitochondrial metabolism. It is a critical component of the mitochondrial fusion machinery and works closely with its homolog Mitofusin 1 (MFN1). Together they form a complex that facilitates the physical merging of mitochondrial membranes. This process is essential for mitochondrial dynamics which include not only fusion but also fission and biogenesis.

Pathways

The protein part of the fusion machinery integrates into multiple essential biological pathways including energy metabolism and apoptosis regulation. It participates in the mitochondrial fusion pathway and the PGC-1α pathway for mitochondrial biogenesis. Mitofusin 2 interacts with proteins such as PINK1 and Parkin that are known to play roles in mitophagy a process that targets damaged mitochondria for degradation indicating its involvement in maintaining mitochondrial quality control.

Mutations in Mitofusin 2 have been linked to Charcot-Marie-Tooth disease type 2A (CMT2A) a neuropathy that affects peripheral nerves. This protein also shows connections to metabolic disorders such as obesity and type 2 diabetes. In these conditions its interaction with other proteins like OPA1 involved in mitochondrial inner membrane fusion influences mitochondrial dysfunction a recognized feature contributing to disease pathogenesis. Understanding MFN2's function and role in disease can help develop targeted therapies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed : 12589796, PubMed : 12598526, PubMed : 14561718, PubMed : 15322553). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (By similarity). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (PubMed : 12598526). Plays an important role in the regulation of vascular smooth muscle cell proliferation (PubMed : 15322553). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria (By similarity). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).

See full target information Mfn2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:423 PubMed37443071

2023

Targeting Wnt/β-catenin-mediated upregulation of oncogenic NLGN3 suppresses cancer stem cells in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jin Yun,Donghwi Kim,Sangwoo Kim,Jer-Tsong Hsieh,Seung Tae Baek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mitofusin 2 antibody [N153/5]

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [N153/5] (AB186317)

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [N153/5] (AB186317)

Western blot - Anti-Mitofusin 2 antibody [N153/5] (AB186317)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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