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Rabbit Recombinant Monoclonal Mitofusin 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 84 publications.


Images

Western blot - Anti-Mitofusin 2 antibody [NIAR164] (AB124773), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (AB124773), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (AB124773), expandable thumbnail
  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (AB124773), expandable thumbnail
  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (AB124773), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300
Notes

For unpurified, use 1/100 - 1/250.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:11181170, PubMed:11950885, PubMed:19889647, PubMed:26214738, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:28114303). Overexpression induces the formation of mitochondrial networks (PubMed:28114303). Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (By similarity). Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) (PubMed:23620051). Is required for PRKN recruitment to dysfunctional mitochondria (PubMed:23620051). Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).

Alternative names

CPRP1, KIAA0214, MFN2, Mitofusin-2, Transmembrane GTPase MFN2

Recommended products

Rabbit Recombinant Monoclonal Mitofusin 2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 84 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
NIAR164
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

This Mitofusin 2 antibody (ab124773) was developed as part of a collaboration between the National Institutes of Health and the lab of Paritosh Ghosh.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Mitofusin 2 also known as MFN2 is a protein involved in the regulation of mitochondrial fusion. The MFN2 molecular weight is roughly 86 kDa. It plays an important role in connecting and merging the outer membranes of mitochondria which is vital for maintaining mitochondrial function and integrity. Mitofusin 2 proteins are expressed in many tissues but they are abundantly present in energy-demanding tissues like skeletal muscle heart and the brain.

Biological function summary

Mitofusin 2 ensures the proper distribution of mitochondria within cells and regulates mitochondrial metabolism. It is a critical component of the mitochondrial fusion machinery and works closely with its homolog Mitofusin 1 (MFN1). Together they form a complex that facilitates the physical merging of mitochondrial membranes. This process is essential for mitochondrial dynamics which include not only fusion but also fission and biogenesis.

Pathways

The protein part of the fusion machinery integrates into multiple essential biological pathways including energy metabolism and apoptosis regulation. It participates in the mitochondrial fusion pathway and the PGC-1α pathway for mitochondrial biogenesis. Mitofusin 2 interacts with proteins such as PINK1 and Parkin that are known to play roles in mitophagy a process that targets damaged mitochondria for degradation indicating its involvement in maintaining mitochondrial quality control.

Associated diseases and disorders

Mutations in Mitofusin 2 have been linked to Charcot-Marie-Tooth disease type 2A (CMT2A) a neuropathy that affects peripheral nerves. This protein also shows connections to metabolic disorders such as obesity and type 2 diabetes. In these conditions its interaction with other proteins like OPA1 involved in mitochondrial inner membrane fusion influences mitochondrial dysfunction a recognized feature contributing to disease pathogenesis. Understanding MFN2's function and role in disease can help develop targeted therapies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773), expandable thumbnail

    Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution

    Lane 1: mouse brain lysate at 20 µg

    Lane 2: mouse kidney lysate at 20 µg

    Lane 3: rat brain lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 86 kDa

    Observed band size: 80 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Immunofluorescence staining of HEK293 cells with purified ab124773 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124773 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab124773 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773), expandable thumbnail

    Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: Jurkat cell lysate at 20 µg

    Lane 3: HEK293 cell lysate at 20 µg

    Lane 4: Raji cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 124 kDa, 86 kDa

    Observed band size: 124 kDa, 80 kDa

  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    All lanes: Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/1000 dilution

    All lanes: Rat primary neurons cell lysate at 20 µg

    Secondary

    All lanes: Anti-rabbit IgG HRP conjugate at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 86 kDa

    Exposure time: 30s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Unpurified ab124773, at 1/50, staining Mitofusin 2 in formalin fixed paraffin embedded Human kidney tissue using immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773), expandable thumbnail

    Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773)

    Western blot: Anti-MFN2 antibody [NIAR164] (ab124773) staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab124773 was shown to bind specifically to MFN2. A band was observed at 64 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in MFN2 knockout cell line. To generate this image, wild-type and MFN2 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: Western blot - Human MFN2 (Mitofusin 2) knockout HEK-293 cell line (Human MFN2 (Mitofusin 2) knockout HEK-293 cell line ab260861)

    Lane 2: MFN2 knockout HEK-293 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: PC-3 cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 64 kDa

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