Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] - BSA and Azide free (AB251421)

This data was developed using ab205235, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 + Mitofusin 1 with ab205235 at 1/50 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] - BSA and Azide free (AB251421)

This data was developed using ab205235, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 + Mitofusin 1 with ab205235 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] - BSA and Azide free (AB251421)

This data was developed using ab205235, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 + Mitofusin 1 with ab205235 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mitochondrial staining on HeLa cells.

The nuclear counter stain is DAPI (blue). Cox IV is detected with ab33985 (anti-Cox IV mouse mAb) at 1/1000 dilution followed by ab150120 (Alexa Fluor^® 594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab205235 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab33985 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] - BSA and Azide free (AB251421)

This data was developed using ab205235, the same antibody clone in a different buffer formulation.

Mitofusin 2 + Mitofusin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab205235 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab205235 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate, 10 μg (Input).
Lane 2 : ab205235 IP in HeLa whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab205235 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] (<a href='/en-us/products/primary-antibodies/mitofusin-2-mitofusin-1-antibody-epr19792-ab205235'>ab205235</a>)

false

• WB

Supplier Data

Western blot - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] - BSA and Azide free (AB251421)

This data was developed using ab205235, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 15 seconds; Lane 3 : 3 seconds.

The expression profile is consistent with the literature (PMID : 14561718; 25574749).

All lanes:

Western blot - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] (<a href='/en-us/products/primary-antibodies/mitofusin-2-mitofusin-1-antibody-epr19792-ab205235'>ab205235</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Observed band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] - BSA and Azide free (AB251421)

This data was developed using ab205235, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Human Mitofusin 2 recombinant protein fragment contains aa151-506 with a His-Tag^®. Human Mitofusin 1 recombinant protein fragment contains aa130-485 with a His-Tag^®.

All lanes:

Western blot - Anti-Mitofusin 2 + Mitofusin 1 antibody [EPR19792] (<a href='/en-us/products/primary-antibodies/mitofusin-2-mitofusin-1-antibody-epr19792-ab205235'>ab205235</a>) at 1/2000 dilution

Lane 1:

Human Mitofusin 2 recombinant protein fragment at 0.01 µg

Lane 2:

Human Mitofusin 1 recombinant protein fragment at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 1s