Mouse Monoclonal Mitotic Cells antibody. Suitable for IHC-Fr, Flow Cyt and reacts with Zebrafish, Human samples. Cited in 2 publications.
Images
![Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (AB8956), expandable thumbnail](https://content.abcam.com/products/images/mitotic-cells-antibody-8b3g-ab8956--flow-cytometry-img90722.gif "Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (AB8956)")
![Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956), expandable thumbnail](https://content.abcam.com/products/images/mitotic-cells-antibody-8b3g-ab8956--immunohistochemistry-frozen-sections-img88636.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)")
![Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956), expandable thumbnail](https://content.abcam.com/products/images/mitotic-cells-antibody-8b3g-ab8956--immunohistochemistry-frozen-sections-img82490.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)")
Publications
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- Translational oncology 12:308-3192018Phosphoproteome Profiling Reveals Multifunctional Protein NPM1 as part of the Irradiation Response of Tumor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadine Wiesmann,Rita Gieringer,Franz Grus,Juergen BriegerPubMed 30453269
- The American journal of pathology 168:878-872006Cell-cycle markers in a transgenic mouse model of human tauopathy: increased levels of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1.Applications:Unspecified applicationReactive species:MousePatrice Delobel,Isabelle Lavenir,Bernardino Ghetti,Max Holzer,Michel GoedertPubMed 16507903
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| IHC-Fr | Flow Cyt | |
|---|---|---|
| Human | Expected | Tested |
| Zebrafish | Tested | Expected |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Recommended products
Mouse Monoclonal Mitotic Cells antibody. Suitable for IHC-Fr, Flow Cyt and reacts with Zebrafish, Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 8B3G
- Purification technique
- Affinity purification Immunogen
- Specificity
Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index.
Together with a quantitative DNA staining procedure (e.g. propidium iodide) this antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle.
Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle.
This antibody can be used for flow cytometric analyses and immunocytochemistry, it is not suitable for immunoblotting.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (ab8956)
Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.
![Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956), expandable thumbnail](https://content.abcam.com/products/images/mitotic-cells-antibody-8b3g-ab8956--immunohistochemistry-frozen-sections-img88636.jpg "Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)")
Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)
ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.
![Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956), expandable thumbnail](https://content.abcam.com/products/images/mitotic-cells-antibody-8b3g-ab8956--immunohistochemistry-frozen-sections-img82490.jpg "Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)")
Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)
ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.
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