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AB8956

Anti-Mitotic Cells antibody [8B3G]

2

(2 Reviews)

|

(2 Publications)

Mouse Monoclonal Mitotic Cells antibody. Suitable for IHC-Fr, Flow Cyt and reacts with Zebrafish, Human samples. Cited in 2 publications.
3 Images
Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (AB8956)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (AB8956)

Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)

ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone : Methanol 1 : 1. ab8956 used at 1 : 50 dilution and incubated for 45 minutes at room temperature.

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (AB8956)

ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone : Methanol 1 : 1. ab8956 used at 1 : 50 dilution and incubated for 45 minutes at room temperature.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8B3G

Isotype

IgM

Carrier free

No

Reacts with

Human, Zebrafish

Applications

IHC-Fr, Flow Cyt

applications

Specificity

Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) this antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle. This antibody can be used for flow cytometric analyses and immunocytochemistry, it is not suitable for immunoblotting.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.</p>" }, "Zebrafish": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 12:308-319 PubMed30453269

2018

Phosphoproteome Profiling Reveals Multifunctional Protein NPM1 as part of the Irradiation Response of Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Wiesmann,Rita Gieringer,Franz Grus,Juergen Brieger

The American journal of pathology 168:878-87 PubMed16507903

2006

Cell-cycle markers in a transgenic mouse model of human tauopathy: increased levels of cyclin-dependent kinase inhibitors p21Cip1 and p27Kip1.

Applications

Unspecified application

Species

Mouse

Patrice Delobel,Isabelle Lavenir,Bernardino Ghetti,Max Holzer,Michel Goedert
View all publications

Product promise

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