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AB308167

Anti-Miz1 antibody [HL1548]

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Rabbit Recombinant Monoclonal Miz1 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ZBTB17.

View Alternative Names

MIZ1, ZNF151, ZNF60, ZBTB17, Zinc finger and BTB domain-containing protein 17, Myc-interacting zinc finger protein 1, Zinc finger protein 151, Zinc finger protein 60, Miz-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Miz1 antibody [HL1548] (AB308167)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Miz1 antibody [HL1548] (AB308167)

Immunofluorescent analysis of 4% paraformaldehyde-fixed U2OS cells labeling Miz1 with ab308167 at 1/500 dilution. Green : Miz1 stained by Miz1 antibody [HL1548] (ab308167) diluted at 1 : 500. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1 : 1000.

Western blot - Anti-Miz1 antibody [HL1548] (AB308167)
  • WB

Supplier Data

Western blot - Anti-Miz1 antibody [HL1548] (AB308167)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Miz1 antibody [HL1548] (ab308167) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Lane 1:

Jurkat cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1548

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ZBTB17. The exact immunogen used to generate this antibody is proprietary information.

Q13105

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Miz1 also known as Zinc finger Miz1 is a transcription factor with a mass of around 87 kDa. It features multiple zinc finger domains allowing it to bind DNA directly. Miz1 is widely expressed in various tissues particularly in the nucleus of cells. Its function includes both activating and repressing gene transcription depending on the context. By modulating transcription Miz1 plays a critical role in cellular proliferation and differentiation.
Biological function summary

Miz1 interacts with other transcription factors and forms part of larger complexes that influence the expression of genes involved in cell cycle regulation. Frequently Miz1 associates with the Myc protein which modifies its activity allowing for tight control over its function. Through these interactions Miz1 regulates important genes that can tip the balance between cell survival and apoptosis.

Pathways

The function of Miz1 integrates into the TGF-beta and MAPK signaling pathways. In the TGF-beta pathway Miz1 collaborates with Smads to regulate target genes that govern cell growth. Meanwhile in the MAPK pathway Miz1's interaction with Myc influences gene expression impacting cellular responses to external signals. These pathways support Miz1's role in controlling cell proliferation and stress responses.

Miz1 demonstrates significant involvement in cancer and developmental disorders. Dysregulation of Miz1 can lead to uncontrolled cell proliferation contributing to oncogenesis. Miz1's interaction with Myc is particularly linked to its oncogenic potential affecting genes that drive tumor progression. Additionally mutations or alterations in Miz1 function can impact developmental processes suggesting its role in some congenital conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Represses RB1 transcription; this repression can be blocked by interaction with ZBTB49 isoform 3/ZNF509S1 (PubMed : 25245946).
See full target information ZBTB17
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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