AB155178

Anti-MK-3 antibody

Name: Anti-MK-3 antibody (ab155178) | Abcam
Brand: Abcam Limited
SKU: AB155178
Price: 495 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal MK-3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAPKAPK3 aa 50 to C-terminus.

View Alternative Names

MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3, MAPKAP-K3, MAPKAPK-3, MK-3, Chromosome 3p kinase, 3pK, MAPKAPK3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-MK-3 antibody (AB155178)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MK-3 antibody (AB155178)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling MK-3 with ab155178 at 1/200 dilution (green). Lower image shows merge with DNA probe (blue).

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Western blot - Anti-MK-3 antibody (AB155178)

10% SDS PAGE

All lanes:

Western blot - Anti-MK-3 antibody (ab155178) at 1/3000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa S3 whole cell lysate at 30 µg

Predicted band size: 43 kDa

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Western blot - Anti-MK-3 antibody (AB155178)

10% SDS PAGE

All lanes:

Western blot - Anti-MK-3 antibody (ab155178) at 1/10000 dilution

All lanes:

Mouse spleen whole cell lysate at 50 µg

Predicted band size: 43 kDa

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Lab

Western blot - Anti-MK-3 antibody (AB155178)

All lanes:

Western blot - Anti-MK-3 antibody (ab155178) at 1/5000 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

MK-3 Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MAPKAPK3 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16644

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MK-3 also known as MAP kinase-activated protein kinase 3 is a serine/threonine kinase with a mass of approximately 43 kDa. It is widely expressed across various tissues including the brain heart and skeletal muscle. Mechanically MK-3 transduces signals from activated MAP kinases and phosphorylates downstream targets influencing their function and stability. This activity is central to many cellular processes particularly those related to responses to external stimuli.

Biological function summary

MK-3 functions as part of the MAPK signaling cascade and phospho-regulates different substrates that influence cell differentiation and stress responses. The protein interacts within complexes that mediate cellular responses to growth factors and various extracellular signals. MK-3's enzymatic activity modulates pathways involved in inflammation and apoptosis which are essential for maintaining cellular homeostasis and responding to stress.

Pathways

MK-3 plays a significant role in the MAPK and SAPK/JNK pathways. These pathways help regulate responses to environmental stressors and cytokine signaling. MK-3 is closely related to other kinases like p38 MAPK and JNK which collaboratively modulate cellular stress responses. These pathways are important for facilitating communication between extracellular stimuli and intracellular actions.

MK-3 has connections to inflammatory conditions and cancer. Inflammatory diseases such as rheumatoid arthritis involve altered MAPK signaling where MK-3 interacts with proteins such as cytokines that promote inflammation. In cancer aberrant MK-3 activity can support tumor progression by affecting apoptosis and cell cycle regulation linking to proteins like p53 that control cell division and death.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally : acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells : required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.

See full target information MAPKAPK3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 30:153-164 PubMed34825496

2021

Combining a β3 adrenergic receptor agonist with alpha-lipoic acid reduces inflammation in male mice with diet-induced obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Zahraa Abdul Sater,Cheryl Cero,Anne E Pierce,Hannah J Lea,Houssein Abdul Sater,Kenneth Y Zhu,Naili Liu,Yinyan Ma,Oksana Gavrilova,Aaron M Cypess

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MK-3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MK-3 antibody (AB155178)

Western blot - Anti-MK-3 antibody (AB155178)

Western blot - Anti-MK-3 antibody (AB155178)

Western blot - Anti-MK-3 antibody (AB155178)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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