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AB250651

Anti-MK-3 antibody [EPR13969] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MK-3 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3, MAPKAP-K3, MAPKAPK-3, MK-3, Chromosome 3p kinase, 3pK, MAPKAPK3

4 Images
Flow Cytometry (Intracellular) - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)

This data was developed using ab183040, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling MK-3 with ab183040 at 1/100 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as Isotype control (green).

Immunoprecipitation - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)
  • IP

Supplier Data

Immunoprecipitation - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)

This data was developed using ab183040, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of Jurkat cells labeling MK-3 using ab183040 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-MK-3 antibody [EPR13969] (<a href='/en-us/products/primary-antibodies/mk-3-antibody-epr13969-ab183040'>ab183040</a>)

Predicted band size: 43 kDa

false

Western blot - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)
  • WB

Supplier Data

Western blot - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)

This data was developed using ab183040, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MK-3 antibody [EPR13969] (<a href='/en-us/products/primary-antibodies/mk-3-antibody-epr13969-ab183040'>ab183040</a>) at 1/5000 dilution

All lanes:

Jurkat cell line lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 43 kDa

false

Western blot - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)
  • WB

Supplier Data

Western blot - Anti-MK-3 antibody [EPR13969] - BSA and Azide free (AB250651)

This data was developed using ab183040, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MK-3 antibody [EPR13969] (<a href='/en-us/products/primary-antibodies/mk-3-antibody-epr13969-ab183040'>ab183040</a>) at 1/20000 dilution

All lanes:

HeLa cell line lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13969

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250651 is the carrier-free version of ab183040.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MK-3 also known as MAP kinase-activated protein kinase 3 is a serine/threonine kinase with a mass of approximately 43 kDa. It is widely expressed across various tissues including the brain heart and skeletal muscle. Mechanically MK-3 transduces signals from activated MAP kinases and phosphorylates downstream targets influencing their function and stability. This activity is central to many cellular processes particularly those related to responses to external stimuli.
Biological function summary

MK-3 functions as part of the MAPK signaling cascade and phospho-regulates different substrates that influence cell differentiation and stress responses. The protein interacts within complexes that mediate cellular responses to growth factors and various extracellular signals. MK-3's enzymatic activity modulates pathways involved in inflammation and apoptosis which are essential for maintaining cellular homeostasis and responding to stress.

Pathways

MK-3 plays a significant role in the MAPK and SAPK/JNK pathways. These pathways help regulate responses to environmental stressors and cytokine signaling. MK-3 is closely related to other kinases like p38 MAPK and JNK which collaboratively modulate cellular stress responses. These pathways are important for facilitating communication between extracellular stimuli and intracellular actions.

MK-3 has connections to inflammatory conditions and cancer. Inflammatory diseases such as rheumatoid arthritis involve altered MAPK signaling where MK-3 interacts with proteins such as cytokines that promote inflammation. In cancer aberrant MK-3 activity can support tumor progression by affecting apoptosis and cell cycle regulation linking to proteins like p53 that control cell division and death.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally : acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells : required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
See full target information MAPKAPK3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neuroreport 33:451-462 PubMed35775321

2022

Notoginsenoside R1 alleviates spinal cord injury by inhibiting oxidative stress, neuronal apoptosis, and inflammation via activating the nuclear factor erythroid 2 related factor 2/heme oxygenase-1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Luo,Zhangli Bao,Mingjian Zhou,Yuxin Chen,Zhaoxi Huang
View all publications
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Product promise

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